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Process for extracting and purifying total flavonoids of Physalis pubescens fruits under ultrasonic assistance

A technology of auxiliary extraction and ultrasonic wave, applied in the direction of cosmetics, cosmetic preparations, plant raw materials, etc., can solve the problems of affecting scientific research and application, large solvent consumption, low efficiency, etc., to maintain antioxidant activity, reduce extraction solvent, consume less Can less effect

Inactive Publication Date: 2017-12-01
JINZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solvent extraction method of flavonoids has low efficiency, large solvent consumption, and has not been purified, so the purity is low, which affects its further scientific research and application

Method used

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  • Process for extracting and purifying total flavonoids of Physalis pubescens fruits under ultrasonic assistance
  • Process for extracting and purifying total flavonoids of Physalis pubescens fruits under ultrasonic assistance
  • Process for extracting and purifying total flavonoids of Physalis pubescens fruits under ultrasonic assistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Single-factor optimization of flavonoids extraction from berry berry

[0022] 1.1 Optimization of solid-liquid ratio

[0023] When the solid-liquid ratio was 1:10, 1:30, 1:50, 1:70, and 1:90 (g / mL), the effect of the solid-liquid ratio on the flavonoid yield and antioxidant activity was investigated, and the experiment was repeated 3 times. . The results showed that the flavonoid yield and antioxidant activity were the best when the solid-liquid ratio was in the range of 1:30-1:70.

[0024] 1.2 Optimization of solvent concentration

[0025] Investigate the effect of solid-liquid ratio on flavonoid yield and antioxidant activity when ethanol concentration is 20%, 40%, 60%, 80%, and 95%, and the experiment is repeated 3 times. The results show that the flavonoid yield and antioxidant activity are the best in the range of ethanol concentration of 60-95%.

[0026] 1.3 Optimization of extraction time

[0027] Investigate the effect of extraction time of 10, 20, 30, 40, ...

Embodiment 2

[0033] Multi-factor optimization of process parameters for extraction of flavonoids from berry berry

[0034] According to the above single-factor experimental results, the three factors of solid-liquid ratio, power and temperature were further optimized, and regression analysis was performed on the data, a response regression model was established, and a quadratic regression equation was obtained by fitting.

[0035] Table 1 Test factor level table

[0036]

[0037] According to the single factor test results, combined with the experimental conditions to select the solid-liquid ratio X 1 , Power X 2 , temperature X 3 It is the three elements of the optimization experiment of flavonoid extraction from Physalis edulis fruit, the fixed ethanol concentration is 80%, and the extraction time is 30min. Experiments were carried out at the levels of factors designed in Table 2, and the experimental results are shown in the table below.

[0038] Table 2 Experimental design schem...

Embodiment 3

[0048] Purification process of total flavonoids in berry fruit by macroporous resin method

[0049] 3.1 The relationship between macroporous resin and flavonoids of Physalis edulis fruit static adsorption and desorption

[0050] Static adsorption and desorption tests were carried out on AB-8 and D101. The results are shown in Table 4. The adsorption rate and desorption rate of AB-8 are higher than those of D101.

[0051] Table 4 Resin adsorption and desorption tests

[0052]

[0053] 3.2 Optimization of sample concentration

[0054] Add resin AB-8 into about 20mL of the chromatographic column (2.0cm×30cm), and prepare 5 parts of sample solutions with different mass concentrations of the crude flavonoid powder of Physalis edulis fruit, the concentrations are 1.0mg / mL, 2.0mg / mL, 3.0mg / mL, 4.0mg / mL and 5.0mg / mL, the sample loading speed is 2mL / min, and the other factors are kept constant. The relationship between the concentration of the sample solution and the adsorption r...

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Abstract

The invention provides a method for extracting and purifying total flavonoids of Physalis pubescens fruits through antioxidant activity tracking and ultrasonic assistance. A certain amount of dried Physalis pubescens fruit powder is mixed with an ethanol solution with the concentration of 60%-95% in a certain solid-to-liquid ratio, extracted for a period of time under ultrasonic assistance at certain power, and an extraction solution is subjected to vacuum concentration; a concentrated solution is subjected to freeze drying treatment, powder is formed, and rough flavonoids of the Physalis pubescens fruits are obtained. The rough flavonoids are purified with AB-8 macroporous adsorption resin, firstly eluted with water for impurity removal and then eluted with an ethanol solution with the concentration of 20%-100%, filtrate is collected, and refined flavonoids of Physalis pubescens are obtained after vacuum concentration and drying. With the adoption of the method, purification is performed 1-3 times, and the purity is increased from 92.7% to 40.02%-65.3%. The flavonoids, prepared with the method, of Physalis pubescens have reducing power and the capability of scavenging negative oxygen ions, hydroxyl radicals and DPPH (diphenyl-picryl hydrazyl) free radicals, and the reducing power and the capability of scavenging negative oxygen ions, hydroxyl radicals and DPPH free radicals are both higher than those of the rough flavonoids and higher than Vc after purification, and the flavonoids of Physalis pubescens can be used for research and development of medicines, healthcare products, food and cosmetics. Extraction and purification of flavonoids are rapid, efficient and convenient, the oxidation resistance of the total flavonoids is reserved to the greatest extent, a basis is provided for industrial production of the flavonoids of Physalis pubescens fruits, and the method has certain practical significance.

Description

technical field [0001] The invention relates to an ultrasonic-assisted extraction and purification process of total flavonoids from berry berries. Background technique [0002] Physalis is an annual dicotyledonous herbaceous plant belonging to the genus Physalis of the family Solanaceae. The fruit is yellow, and its fruit is rich in flavonoids, withanolide and other active ingredients. The solvent extraction method of flavonoids is inefficient, consumes a lot of solvent, and has not been purified, so the purity is low, which affects its further scientific research and application. Therefore, it is of practical significance to develop a fast, effective, and low-consumption extraction and purification method, and to retain its maximum antioxidant capacity, to provide a material and theoretical basis for the comprehensive development of this plant as a medicinal and edible resource. Contents of the invention [0003] In order to solve the above-mentioned existing problems, ...

Claims

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Application Information

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IPC IPC(8): A61K36/81A61P39/06A61K8/9789A61Q19/08A23L33/105A61K131/00
CPCA23L33/105A23V2002/00A61K8/97A61K36/81A61K2236/333A61K2236/51A61K2236/55A61Q19/08A23V2200/302
Inventor 王晶晶杜玉慧张振
Owner JINZHOU MEDICAL UNIV
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