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A synergistic anti-tumor polyphenol composition and its application

A polyphenol composition and a synergistic technology, applied in the field of medicine, can solve the problems of organ damage, damage to normal cells, low immune function, etc., and achieve the effect of reducing the dose and reducing the occurrence of drug resistance of tumor cells

Active Publication Date: 2020-04-28
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemotherapy is one of the most important and effective means of treating tumors today. When it acts on target cells, it often damages normal cells, causing organ damage, low immune function, bone number suppression and other toxic side effects. Tumor cells will also develop problems such as multidrug resistance, which greatly limits the efficacy

Method used

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  • A synergistic anti-tumor polyphenol composition and its application
  • A synergistic anti-tumor polyphenol composition and its application
  • A synergistic anti-tumor polyphenol composition and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A synergistic anti-tumor polyphenol composition is a composition of quercetin and alfalfa.

[0025] The enhancing effect of quercetin combined with alfalfa in inhibiting the proliferation of human breast cancer MCF-7 cells (purchased from ATCC, USA) in vitro:

[0026] Human breast cancer MCF-7 cells of passage 10-30 were selected. Remove the cell culture medium (DMEM, purchased from Hyclone Company, USA), and wash with cold PBS. Then, the adherent cells were digested with trypsin (purchased from Gibco, USA). The digestion time was 2 min, and 4 mL of wash medium (purchased from Hyclone, USA) was added to terminate the digestion. Use a pipette to transfer the culture solution to a centrifuge tube, and centrifuge at 760 rpm for 4-5 minutes at a temperature of 4°C. cells in 2.5 x 10 4 The density of cells / well was spread in a 96-well plate, 100 μL per well. at 5% CO 2After culturing at 37°C for 6 hours in the incubator, 100 μL of a composition solution of quercetin an...

Embodiment 2

[0033] A synergistic anti-tumor polyphenol composition is a composition of luteolin and alfalfa.

[0034] Enhancement effect of luteolin combined with alfalfa in inhibiting the proliferation of human breast cancer MCF-7 cells in vitro:

[0035] Human breast cancer MCF-7 cells (purchased from ATCC, USA) of passage 10-30 were selected. Remove the cell culture medium (DMEM, purchased from Hyclone Company, USA), and wash with cold PBS. Then, the adherent cells were digested with trypsin (purchased from Gibco, USA). The digestion time was 2 min, and 4 mL of wash medium (purchased from Hyclone, USA) was added to terminate the digestion. Use a pipette to transfer the culture solution to a centrifuge tube, and centrifuge at 760 rpm for 4-5 minutes at a temperature of 4°C. cells in 2.5 x 10 4 The density of cells / well was spread in a 96-well plate, 100 μL per well. at 5% CO 2 After culturing for 6 hours at 37°C in the incubator, add 100 μL of the composition solution of luteolin ...

Embodiment 3

[0042] A synergistic anti-tumor polyphenol composition is a composition of p-coumaric acid and luteolin.

[0043] Synergistic effect of p-coumaric acid combined with luteolin in inhibiting the proliferation of human breast cancer MCF-7 cells in vitro:

[0044] Human breast cancer MCF-7 cells (purchased from ATCC, USA) of passage 10-30 were selected. Remove the cell culture medium (DMEM, purchased from Hyclone Company, USA), and wash with cold PBS. Then, the adherent cells were digested with trypsin (purchased from Gibco, USA). The digestion time was 2 min, and 4 mL of wash medium (purchased from Hyclone, USA) was added to terminate the digestion. Use a pipette to transfer the culture solution to a centrifuge tube, and centrifuge at 760 rpm for 4-5 minutes at a temperature of 4°C. cells in 2.5 x 10 4 The density of cells / well was spread in a 96-well plate, 100 μL per well. at 5% CO 2 After culturing for 6 hours at 37°C in the incubator, add 100 μL of a composition solutio...

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Abstract

The invention belongs to the technical field of drugs, food and health products, and discloses synergistic anti-tumor polyphenol composition and application. The polyphenol composition is the composition of two or more of tricin, quercetin, luteolin and p-coumaric acid, preferably, the composition of tricin and quercetin, the composition of tricin and luteolin, or the composition of p-coumaric acid and luteolin. The composition has obvious synergistic effect of resisting tumor cell proliferation, the effect is better than that of an independently used polyphenol compound, the dose of a drug can be reduced, and drug resistance of tumor cells is reduced. The polyphenol composition is used for preparing anti-tumor drugs, food, health products and functional food additives.

Description

technical field [0001] The invention belongs to the technical field of medicines, and relates to a synergistic anti-tumor composition and its application, in particular to an anti-tumor polyphenol composition. The anti-tumor refers to inhibiting the proliferation of human breast cancer MCF-7 cells. Background technique [0002] Today, cancer has become the number one disease that causes human death. The morbidity and mortality of cancer are increasing year by year in China. Since 2010, cancer has become the most lethal disease in my country, and it is also one of the most important public health problems in China. According to statistics, the incidence of cancer in Chinese residents has generally shown an upward trend in the past ten years, and the incidence of breast cancer ranks first in urban and rural female residents, and it is one of the most important malignant tumors that endanger the life and health of Chinese residents (Chen Wanqing, Zheng Rongshou. China Mortalit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/352A61K31/192A61P35/00A23L33/105
CPCA23L33/105A23V2002/00A61K31/192A61K31/352A61K2300/00A23V2200/308A23V2250/21168A23V2250/2132
Inventor 赵振刚郑瑞刘瑞海
Owner SOUTH CHINA UNIV OF TECH
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