Food-grade fluorescent nano-microemulsion and preparation method thereof
A fluorescent nano, food-grade technology, applied in the field of preparation of fluorescent nano-microemulsions, can solve the problems of undiscovered optical properties of fluorescent micro-emulsions, etc., and achieve simple and easy-to-obtain raw materials, good biocompatibility, and low preparation costs Effect
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Embodiment 1
[0028] A preparation method for food-grade fluorescent nano-microemulsion, comprising the steps of:
[0029] (1) Mix 90mg of isoamyl acetate and 30mg of absolute ethanol to obtain a mixed oil phase, then add 1.5 times the quality of the mixed oil phase, that is, 180mg CrEL, mix it in a small beaker, add the rotor, adjust the rotating speed to 150rpm, Stir for 10 minutes at 4°C;
[0030] (2) At a stirring speed of 150rpm, add water dropwise, the total mass of the added water is 1.5 times the total mass of the liquid obtained in step (1), to obtain a uniform, transparent food-grade fluorescent nano-microemulsion.
[0031] The particle diameter of the obtained microemulsion is 26.34nm, the PDI (polydispersity Index) is 0.309, and the fluorescence intensity is near 8000.
Embodiment 2
[0033] A preparation method for food-grade fluorescent nano-microemulsion, comprising the steps of:
[0034] (1) Mix 90mg of isoamyl acetate and 60mg of absolute ethanol to obtain a mixed oil phase, then add 3 times the mass of the mixed oil phase, that is, 450mg CrEL, and mix it in a small beaker, add the rotor, adjust the rotating speed to 200rpm, Stir for 20min at 25°C;
[0035] (2) At a stirring speed of 200rpm, add water dropwise, and the total mass of the added water is 2.33 times the total mass of the liquid obtained in step (1); obtain a uniform, transparent food-grade fluorescent nano-microemulsion.
[0036] The particle diameter of the obtained microemulsion is 25.62nm, the PDI (polydispersity Index) is 0.323, and the fluorescence intensity is near 8200.
Embodiment 3
[0038] A preparation method for food-grade fluorescent nano-microemulsion, comprising the steps of:
[0039] (1) Mix 40mg of isoamyl acetate and 40mg of absolute ethanol to obtain a mixed oil phase, then add 6 times the mass of the mixed oil phase, that is, 480mg CrEL, mix in a small beaker, add the rotor, adjust the rotating speed to 250rpm, Stir for 30min at 50°C;
[0040] (2) At a stirring speed of 250rpm, add water dropwise, and the total mass of the added water is 4 times the total mass of the liquid obtained in step (1); obtain a uniform and transparent food-grade fluorescent nano-microemulsion.
[0041] The particle diameter of the obtained microemulsion is 23.24nm, the PDI (polydispersity Index) is 0.378, and the fluorescence intensity is near 7500. Example 4
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