A method for screening oyster parents with high glycogen content and related primer pairs

Abstract

The invention relates to a method for screening out crassostrea gigas high-glycogen content parent oyster and a primer pair of an SNP marker related to the same. An SNP locus has A and G allele modes in a genome DNA; upstream and downstream 500bp nucleotide sequences are shown in SEQ ID No.1. An operational result shows that by means of the method, the SNP locus can be accurately detected, and a high-glycogen content individual is screened out; meanwhile, compared with an AA genotype individual, the glycogen content of the GG genotype individual of the locus is remarkably increased by 4.5%. In the follow-up processes, according to the method, the GG genotype parent oyster is screened out, and oyster breeding is guided. The invention provides development and potential application of the SNP marker for screening out the crassostrea gigas high-glycogen content individual. The method has the advantages that genotype identification can be performed on the parent oyster before breeding, and the filial generation glycogen content is increased. According to research results, the reliability of the SNP marker is higher, the range of adaption community is wider, and the effect is more stable.

Description

technical field [0001] The invention belongs to the field of genetic engineering and genetic breeding, and relates to a method for screening oyster parents with high glycogen content and a pair of primers related to SNP markers. Background technique [0002] Oysters are an important marine aquatic resource and one of the most important marine cultured shellfish in the world. my country's oyster farming scale and output have been ranked first in the world for many years, but for a long time, my country's oysters can only be self-produced and sold, and it is difficult to enter the high-end market. Tracing it to its cause is mainly due to only paying attention to the high yield and high quantity of oysters in the breeding and breeding process, ignoring the content of its nutritional quality. The high content of nutrients such as glycogen is one of the most important characteristics of oysters. It not only affects the plumpness and yield of oysters, but also affects the taste o...

AI Technical Summary

Problems solved by technology

In the existing research on shellfish, the development of molecular markers mainly focuses on the known functional genes. The relative number of markers is relatively small, and the main effect sites may not be obtained, and it is impossible to have a comprehensive understanding of the genetic regulation mechanism of phenotypes.

So far, there is no report on the main effect loci based on genome-wide association analysis

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