Breeding method of brassica napus radish cytoplasm sterile restorer line and application to brassica napus breeding

Abstract

The invention relates to a breeding method of a brassica napus radish cytoplasm sterile restorer line. Through brassica napus radish cytoplasm sterile restorer material CLR650 whole genome resequencing data and radish genome sketch information comparison, specific molecular markers of linked exogenous radish fragments and restoring genes in CLR650 are developed; then, a target single strain of the brassica napus radish cytoplasm sterile restorer material CLR650 backcross population is bred in an auxiliary way through the specific molecular markers; a BC4 separation group is obtained; fertile plants in the BC4 group is subjected to multi-generation mixed selection by a mixed method until the mixed separation group with the fertile plant proportion exceeding 75 percent is obtained; finally, the homozygosis stabilization is performed through multi-generation selfing; the novel brassica napus radish cytoplasm sterile restorer line is obtained. The method provided by the invention overcomes the defects of too low restoring gene heritability and property homozygosis stabilization difficulty of the existing method.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding, and in particular relates to a method for breeding a cytoplasmic sterile restorer line of Brassica napus radish based on hybridization, sister crossing, selfing conventional breeding techniques and molecular marker-assisted selection techniques. Background technique [0002] Rapeseed has obvious heterosis, and is the most potential oil crop at home and abroad to cope with the shortage of edible vegetable oil and develop renewable bioenergy. The radish cytoplasmic male sterility ogu CMS is a cytoplasmic male sterility system established by Ogura, Japan in 1968, which found the sterility source in radish. Because the sterility of this system is very stable and thorough, it can be used in hybrids of cruciferous crops. It has very important value in the utilization of dominance, and it is already an ideal way to utilize heterosis in Brassica napus. But because its restorer gene exists only...

AI Technical Summary

Problems solved by technology

For example, Li Xufeng reported that the restorer gene was introduced from the radish variety Lanhuazi into rapeseed, but the restorative genes of the obtained materials were all on the episomes and could not be stably inherited; Liu Xuanxia used the existing B. A new restorer line with improved agronomic traits was screened out by crossing with approved winter rapeseed varieties, but the research was only conventional crossing and homozygous selection, and the source of the restorer could not be determined

Because it is very difficult to transfer restorer lines, my country has always lacked available restorer sources

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