SST2 detection kit based on microparticle chemical luminescence immunity analysis

Abstract

An sST2 detection kit based on microparticle chemiluminescence immunoassay relates to a chemiluminescence detection kit. The sST2 detection kit is equipped with reaction buffer, magnetic particles coated with sST2 monoclonal antibody, sST2 monoclonal antibody labeled with acridinium ester, luminescent solution, sST2 antigen standard solution, and concentrated washing solution. Prepare reaction buffer; prepare magnetic particles coated with sST2 antibody; prepare acridinium ester-labeled sST2 antibody; prepare sST2 antigen standard solution; prepare luminescent solution; prepare washing solution. It adopts microparticle chemiluminescence immunoassay technology, which has higher sensitivity and better stability than ELISA, filling the gap in the domestic production of microparticle chemiluminescence diagnostic reagents for human serum sST2 detection. It has the advantages of simple operation, high sensitivity, wide linear range, stable results, good safety, and easy automation. It has broad application prospects in clinical testing and other aspects.

Description

technical field [0001] The invention relates to a chemiluminescence detection kit, in particular to a sST2 detection kit based on microparticle chemiluminescence immunoassay. Background technique [0002] Heart failure refers to a group of complex clinical syndromes in which any structural or functional abnormality of the heart results in impaired filling or ejection ability of the heart, and is the terminal stage of various heart diseases. At present, there are nearly 23 million people suffering from heart failure in the world, of which nearly 6 million people in the United States suffer from heart failure, and nearly 5 million people in China also suffer from heart failure, accounting for about 1 / 4 of the total number of people in the world. NT-proBNP is currently recognized as an effective indicator for the diagnosis of heart failure. It is widely used because of its high sensitivity, positive correlation with the degree of heart failure, and ability to predict the treat...

AI Technical Summary

Problems solved by technology

However, NT-proBNP has the following shortcomings as a marker of heart failure: NT-proBNP will increase with age, and women are higher than men; other diseases such as acute coronary syndrome, lung disease, and chronic renal insufficiency can also lead to elevated NT-proBNP

At present, microparticle chemiluminescence immunoassay has been widely used in the detection of various antigens such as hepatitis B two-and-a-half (CN 102072957), sex hormones (CN103954779), tumor markers, etc., and has achieved good social benefits, but there is no such method at home and abroad. The sST2 detection kit comes out, the research and development of this project will fill this gap, and its market application prospect is broad

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