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Detection method and device

A detection method and detection device technology, applied in special data processing applications, instruments, electrical digital data processing, etc., can solve the problems of high-throughput sequencing data sample contamination detection, avoid difficulty and uncertainty, and achieve accurate Detect and improve the effect of accuracy

Active Publication Date: 2017-10-20
AEGICARE (SHENZHEN) TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of this, the embodiment of the present invention provides a detection method and device to solve the problem in the prior art that high-throughput sequencing data cannot be detected for sample contamination

Method used

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Experimental program
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Embodiment 1

[0022] figure 1 It is a flow chart of a detection method provided in Embodiment 1 of the present invention. The execution subject of the embodiment of the present invention is a computing device, which may specifically be a computer, a server, etc. figure 1 An exemplary detection method may specifically include steps S101 to S103, which are described in detail as follows:

[0023] S101. Obtain result data obtained by performing high-throughput sequencing on a sample.

[0024] High-throughput sequencing (High-throughput sequencing), also known as "Next-generation" sequencing technology, can perform sequence determination and general read length on hundreds of thousands to several million DNA molecules in parallel at a time. Shorter is the flag.

[0025] Specifically, a set of result data is obtained by performing high-throughput sequencing on the samples.

[0026] S102. According to the result data, the Bayesian method is used to calculate the probability set of the pollutio...

Embodiment 2

[0034] figure 2 It is a flow chart of a detection method provided in Embodiment 2 of the present invention. The execution subject of the embodiment of the present invention is a computing device, which may specifically be a computer, a server, etc. figure 2 An exemplary detection method may specifically include steps S201 to S206, which are described in detail as follows:

[0035] S201. Obtain result data obtained by performing high-throughput sequencing on a sample.

[0036] High-throughput sequencing, also known as "next-generation" sequencing technology, is marked by the ability to sequence hundreds of thousands to millions of DNA molecules in parallel at a time and generally having a short read length.

[0037] Specifically, a set of result data is obtained by performing high-throughput sequencing on the samples.

[0038] S202. List the contamination rates of the samples according to the fixed step size.

[0039] Specifically, the value range of the pollution rate of ...

Embodiment 3

[0064] image 3 It is a schematic structural diagram of a detection device provided in Embodiment 3 of the present invention. For convenience of description, only the parts related to the embodiment of the present invention are shown. image 3 An exemplary detection device may be the subject of execution of the detection method provided in Embodiment 1 above. image 3 A detection device of an example includes: an acquisition module 31, a statistical module 32 and a determination module 33, and each functional module is described in detail as follows:

[0065] An acquisition module 31, configured to acquire the result data obtained by performing high-throughput sequencing on the sample;

[0066] Statistical module 32, is used for according to the result data that acquisition module 31 obtains, adopts Bayesian method to count the probability collection of the pollution rate of sample;

[0067] The determining module 33 is configured to determine the contamination level of the ...

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Abstract

The invention is suitable for the technical field of molecular biological information detection, and provides a detection method and device. The method comprises the following steps of: obtaining result data which is obtained through carrying out high-throughput sequencing on samples; carrying out statistic on a probability set of contamination rates of the samples by adoption of a Bayes method according to the result data; and determining the contamination levels of the samples according to the probability set. According to the method and device provided by the invention, the correct detection for the sample contamination levels of samples of high-throughput sequenced data is realized, the difficulty and uncertainty caused by carrying out sample pollution control before sequencing are avoided, and the correctness of later-period analysis results is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of molecular biological information detection, and in particular relates to a detection method and device. Background technique [0002] When high-throughput sequencing is used for gene detection of genetic diseases, a large number of short-read sequences will be generated for deoxyribonucleic acid (DNA) sequencing and gene mutation detection. Contamination has a great impact on high-throughput sequencing. Even a small amount of contamination can lead to inaccurate test results, false positive or false negative results. [0003] In the existing high-throughput sequencing process, the occurrence of cross-contamination is often reduced through the previous experimental process and sample processing, but it is difficult and uncertain to control cross-contamination in this way, due to the sequencing depth and calculation Due to the limitations of the method, there is currently no direct cross-contamination dete...

Claims

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Application Information

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IPC IPC(8): G06F19/20G06F19/22
CPCG16B25/00G16B30/00
Inventor 李阳
Owner AEGICARE (SHENZHEN) TECH CO LTD
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