Spectral microscopic imaging method and system thereof

Abstract

The invention relates to a snapshot spectral microscopic imaging method and a system thereof. For providing lossless snapshot spectral microscopic imaging technology, a substance structure or a component image of a living body is tracked or snapshot in real time. For this purpose, the imaging method and the system thereof are characterized in that the spectral microscopic imaging system is composed of a front optical system imaging part, a transit lens, an image splicer, an imaging lens set, an imaging spectrograph and a spectral image fusion reconstruction module. Imaging is performed on a to-be-imaged object by the front optical system; a formed image is transmitted to the image splicer through the transit lens; through cutting and reflection of the image splicer, the image arrives at the imaging lens set; the imaging lens set performs integration on the cut sub-images for realizing regular arrangement on a slit inlet of the imaging spectrograph. Finally a whole three-dimensional data set is obtained through the spectral image fusion reconstruction module. The spectral microscopic imaging method and the system thereof are mainly used for a snapshot spectral microscopic imaging occasion.

Description

technical field [0001] The invention relates to a snapshot-type spectral microscopic imaging method and system, in particular to a spectral microscopic imaging method and system capable of obtaining all spectral images in one exposure. Specifically, it relates to a spectral microscopic imaging method and system. Background technique [0002] Microspectroscopy is an indispensable tool in modern life science research. However, most of the existing microspectrometers are scanning, such as multispectral laser confocal microscopic imaging, based on Acousto-optic Tunable Filter (AOTF), microscopic interference polarization imaging spectrometer and liquid crystal tunable filter. (Liquid Crystal Tunable Filters, LCTF). The multi-spectral laser confocal microscopy imaging method is to set two movable slits at the spectral surface to form an exit slit, and at the same time, two stepping motors drive the two slits installed on it to scan in order to obtain Spectral images within the...

AI Technical Summary

Problems solved by technology

Like CTIS, its image restoration calculation is very complicated

[0004] An existing Snapshot ImageMapping Spectrometer (IMS) system based on Integral Field Spectrometry (IFU) obtains spectral information and spatial information by segmenting the image formed by a micro-segmented array microscope, and then disperses it, but introduces a large number of lenses into the device , it is inevitable to produce problems such as chromatic aberration and loss of light energy in the device. At the same time, because the cutting part of the device uses a flat mirror, the integration level is not high

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