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Microalgae medium and method for culturing microalgae to produce hydrogen

A technology for producing hydrogen and culture medium, which is applied in the fields of bioengineering and medicine, can solve the problems of low hydrogen production and hydrogen production rate, and achieve the goal of increasing hydrogen production and hydrogen production rate, increasing hydrogenase activity, and enhancing cellular respiration Effect

Active Publication Date: 2017-10-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0006] The present invention aims at the problem of low hydrogen production and hydrogen production rate existing in the prior art by constructing chlorella aggregates to continuously produce hydrogen, and provides a microalgae culture that improves hydrogen production and hydrogen production rate Base and method for cultivating microalgae to produce hydrogen using the microalgae culture medium

Method used

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  • Microalgae medium and method for culturing microalgae to produce hydrogen
  • Microalgae medium and method for culturing microalgae to produce hydrogen
  • Microalgae medium and method for culturing microalgae to produce hydrogen

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Experimental program
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Embodiment 1

[0045] Chlorella cells were cultured to logarithmic phase (cell density 1×10 8cells / mL), adding 1 g / L cationic polyelectrolyte polydimethyldiallylammonium chloride (PDADMAC) to modify the surface of algal cells, so that the algal cells whose surface was originally negatively charged were positively charged. Then, the algal liquid collected by centrifugal washing was evenly mixed with 5 mM sodium silicate solution, and stirred for 30 min to form microalgae aggregates. Then wash and resuspend with TAP medium, and divide into 60mL sealed tubes with 30mL algae solution in each tube, and the above is used as the control group. Add DMSO to the above 30mL algae liquid to make the final concentration 0.5% (volume ratio), as the experimental group. The two groups were at 100μE m -2 the s -1 Exposure to light for 48 hours.

[0046] (1) The aggregated chlorella and the chlorella aggregates added with DMSO were observed under an optical microscope, and the particle size statistical an...

Embodiment 2

[0050] Chlorella cells were cultured to logarithmic phase (cell density 1×10 8 cells / mL), adding 1g / L cationic PDADMAC to modify the surface of the algal cells, so that the algal cells whose surface was originally negatively charged were positively charged. Then, the algal liquid collected by centrifugal washing was evenly mixed with 5 mM sodium silicate solution, and stirred for 30 min to form microalgae aggregates. Then wash and resuspend with TAP medium, and divide into 60mL sealed tubes with 30mL algae solution in each tube, and the above is used as the control group. Then add DMSO to the above 30mL algae liquid, so that the final concentrations are 0.1%, 0.3%, 0.5%, 1%, 2%, 3%, 5% (volume ratio), as the experimental group. respectively at 100μE m -2 the s -1 After irradiating under light for 48 hours, measure the hydrogen accumulation in the headspace of the sealed tube. Each concentration was repeated 5 times.

[0051] The result is as Figure 4 As shown in Table 1...

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Abstract

The invention discloses a microalgae medium and a method for culturing microalgae to produce hydrogen. The microalgae medium contains a basic medium and an additive, the additive is DMSO, and the adding amount of DMSO is 0.1%-2% according to a volume ratio. The method comprises the steps of: (1) constructing a microalgae aggregate; and (2) resuspending the microalgae aggregate in the microalgae medium, and performing culture under an illumination condition to produce hydrogen. According to the microalgae medium provided by the invention, a certain amount of DMSO is added into an ordinary medium, when the microalgae medium is applied in culturing microalgae to produce hydrogen, cellular respiration is enhanced, so that more layers of cells in the aggregate are in an oxygen-free environment and participate in hydrogen production, the hydrogenase activity is enhanced, and the hydrogen yield and hydrogen production rate are also effectively improved.

Description

technical field [0001] The invention relates to the field of bioengineering and technology, in particular to a microalgae culture medium and a method for cultivating microalgae to produce hydrogen. Background technique [0002] Hydrogen is considered as an ideal alternative to fossil energy due to its non-polluting, renewable and high energy conversion efficiency. At present, the most important hydrogen production methods are petroleum cracking, photocatalytic decomposition or electrolysis of water and biological hydrogen production. Among them, biological hydrogen production, especially photosynthetic hydrogen production, is environmentally friendly, and its main energy source is solar energy, while other hydrogen production methods consume huge amounts of energy. In nature, photosynthetic microalgae can photolyze water through photosynthetic system 2 to generate oxygen and photosynthetic electrons, and then the electrons are transferred to hydrogenase to catalyze the gene...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P3/00C12R1/89
CPCC12N1/12C12P3/00
Inventor 舒蕾马为民徐旭荣唐睿康
Owner ZHEJIANG UNIV
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