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Drug-resistant estrogen-related receptor gene LSERR of laodelphax striatellus, gene segment capable of reducing drug resistance of laodelphax striatellus and application of gene segment

A technology of receptor genes and gene fragments, applied in the direction of hormone receptors, DNA/RNA fragments, receptors/cell surface antigens/cell surface determinants, etc., can solve the problem of pest death, abnormal development and reproduction of pests, lethal toxicity And other issues

Active Publication Date: 2017-10-20
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the current actual situation, the public generally has a deep misunderstanding of genetically modified technology, and it is difficult to accept genetically modified crops directly; dsRNA is easily misunderstood by the public as the dsRNA itself has lethal toxicity, or developmental / reproductive toxicity, making this dsRNA not easily accepted

Method used

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  • Drug-resistant estrogen-related receptor gene LSERR of laodelphax striatellus, gene segment capable of reducing drug resistance of laodelphax striatellus and application of gene segment
  • Drug-resistant estrogen-related receptor gene LSERR of laodelphax striatellus, gene segment capable of reducing drug resistance of laodelphax striatellus and application of gene segment
  • Drug-resistant estrogen-related receptor gene LSERR of laodelphax striatellus, gene segment capable of reducing drug resistance of laodelphax striatellus and application of gene segment

Examples

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specific Embodiment

[0076] The present invention will be further described below by way of examples, but the present invention is not limited to these examples.

[0077] The sulfoxaflor-resistant strain used in the present invention was collected from the experimental field of the Jiangsu Academy of Agricultural Sciences in Nanjing, Jiangsu in 2010, and was obtained by continuous screening with sulfoxaflor for 35 generations; the sensitive strain was collected from the Jiangsu Academy of Agricultural Sciences in Nanjing, Jiangsu in 2010 The experimental field was obtained by continuous breeding indoors without exposure to any pesticides.

[0078] The reagents involved in the present invention are all commercially available.

[0079] The experimental methods of the following examples are conventional methods unless otherwise specified.

Embodiment 1

[0080] Cloning and sequence analysis of embodiment 1.LSERR gene

[0081] The present invention obtains transcriptome data by constructing a transcriptome library of SBPH and performing high-throughput sequencing, and obtains LSERR gene fragments on this basis.

[0082] (1) Extraction of total RNA from SBPH.

[0083] Using sensitive strains of SBPH as research materials, select SBPH eggs, 1-5 instar nymphs, and male and female adults for mixed extraction using TRIzol Total RNA Extraction Kit (Shanghai Yingjun Biotechnology Co., Ltd.), and the operation method refers to the reagents box instructions. The extracted total RNA of SBPH was electrophoresed on agarose gel with a concentration of 1.0%, and the RNA concentration and absorbance value were detected with a Nanodrop1000 nucleic acid protein quantifier to confirm the quality and integrity of the RNA, and then placed in an ultra-low temperature refrigerator at Freeze at -80°C.

[0084] (2) Preparation of cDNA template.

...

Embodiment 2

[0112] Example 2. Semi-quantitative analysis of LSERR gene in sulfoxaflor-resistant and sensitive strains of SBPH

[0113] The sulfoxaflor-resistant and sensitive SBPH materials in the adult stage were selected, and the expression level of LSERR gene in these materials was analyzed by semi-quantitative RT-PCR, so that the relationship between the expression level of LSERR gene and the resistance to sulfoxaflor could be quickly obtained. Drug relationship.

[0114] (1) Extraction of total RNA from SBPH.

[0115] Using sulfoxaflor-resistant and sensitive SBPH in the adult stage as materials, total RNA was extracted using the SV Total RNA extraction kit (Promega (Beijing) Biotechnology Co., Ltd.) according to the operating procedures, and the extracted ash The total RNA of planthoppers was electrophoresed on agarose gel with a concentration of 1.0%, and the concentration and absorbance of the RNA were detected with a Nanodrop100 nucleic acid protein quantifier to confirm the qua...

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Abstract

The invention relates to a drug-resistant estrogen-related receptor gene LSERR of laodelphax striatellus, a gene segment capable of reducing drug resistance of laodelphax striatellus and an application of the gene segment. The sequence of the drug-resistant estrogen-related receptor gene LSERR of laodelphax striatellus is shown as SEQ ID NO.1. The sequence of the gene segment capable of reducing drug resistance of laodelphax striatellus is shown as SEQ ID NO.3. The gene segment is used for reducing drug resistance of laodelphax striatellus. The gene segment can be used for detecting drug resistance of laodelphax striatellus, and drug resistance management of laodelphax striatellus is realized.

Description

technical field [0001] The invention relates to a drug-resistant estrogen-related receptor gene LSERR of the planthopper, a gene fragment for reducing the drug resistance of the planthopper and an application thereof, and belongs to the field of biotechnology. Background technique [0002] As far as the inventor knows, in my country, SBPH is distributed in various provinces and cities, and occurs more in the Yangtze River Basin and North China rice fields. The most important form of harm of SBPH is that population outbreaks promote the prevalence of plant virus diseases and cause huge economic losses. At present, chemical pesticides are mainly used to control SBPH, which has resulted in different levels of resistance of SBPH to various commonly used insecticides. It is urgent to monitor the resistance of SBPH and study the mechanism of resistance formation. Develop management techniques and strategies to achieve sustainable control of SBPH. [0003] The inventor finds out ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/72C12N15/12C12N15/113C12N15/10A01K67/033
CPCA01K67/033C07K14/721C12N15/10C12N15/1138C12N2310/14C12N2330/50C12Q2531/113
Inventor 徐鹿顾中言李海东许小龙徐德进徐广春韩召军
Owner JIANGSU ACAD OF AGRI SCI
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