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A nutritional method to regulate the secretion of sex hormones in half-smooth tongue sole broodstock

A technology for hormone secretion and semi-smooth tongue sole, which is applied in the field of nutrition regulating the secretion of sex hormones of semi-smooth tongue sole broodstock, can solve the problems of neglecting precise demands, declining reproductive performance, unreasonable nutritional supply, etc., and achieves the effects of high economy and strong operability.

Active Publication Date: 2019-09-27
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current breeding of half-smooth tongue sole broodstock, most of them use the extensive feeding method of fresh miscellaneous fish, ignoring the precise needs of various nutrients for half-smooth tongue sole broodstock, which leads to unreasonable nutrient supply in the broodstock breeding stage
On the other hand, in different stages of broodstock gonad development, in fact, their nutritional requirements are not the same, and in actual production, this point is often overlooked. The single nutritional strategy from beginning to end in the broodstock breeding process violates the broodstock gonad development process. The changing laws of physiological metabolism and nutritional requirements in the body lead to the decline of reproductive performance

Method used

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  • A nutritional method to regulate the secretion of sex hormones in half-smooth tongue sole broodstock
  • A nutritional method to regulate the secretion of sex hormones in half-smooth tongue sole broodstock
  • A nutritional method to regulate the secretion of sex hormones in half-smooth tongue sole broodstock

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1. Screening experiment of DHA and EPA addition amount in the feed of male and female semi-smooth tongue sole broodstock at different gonadal development stages (fixed ARA content)

[0024] 1. Experimental design and experimental feed formula

[0025] Fishmeal, casein, and high-gluten flour are used as the main protein source, and soybean lecithin, olive oil, and soybean oil are used as the main fat source to formulate the basic feed. Add DHA and EPA in the basic feed, and prepare 3 kinds of isonitrogen and fat feeds (protein content 57.8%, fat content 12.5%) with different DHA and EPA levels, and make the total amount of (DHA+EPA) in the feed account for 2.8% of the content. At the same time, a certain amount of ARA was added to the feed to meet the needs of the experimental fish for n-6 long-chain polyunsaturated fatty acids (n-6LC-PUFA). The feed formulation and fatty acid composition are shown in Table 1 and Table 2, respectively.

[0026] Table 1 Feed fo...

Embodiment 2

[0047] Example 2. Screening experiment of ARA addition amount in feed of male and female semi-smooth tongue sole broodstock at different gonadal development stages (fixed DHA and EPA content)

[0048] 1. Experimental design and experimental feed formula

[0049] Fishmeal, casein and wheat flour were used as the main protein sources, and soybean lecithin, olive oil, tristearin, etc. were used as the main fat sources to prepare 5 kinds of isonitrogen and isolipid experimental feeds with different ARA levels (protein content 55.5%, fat content 13%). ARA purified oil (ARA content, 41% of total fatty acids; triglyceride form; Jiangsu Tiankai Biotechnology Co., Ltd., Nanjing, China) was used to replace glyceryl tristearate in the control group to make 5 experimental group feeds, The ratios of ARA to total fatty acids in feed were 0.6%, 2.0%, 5.1%, 10.3% and 15.4%, respectively. And a certain amount of n-3LC-PUFA (containing 37% DHA and 21% EPA, triglyceride form; Hebei Haiyuan Kan...

Embodiment 3

[0074] Example 3, the overall application of the technical solution of the present invention in the breeding experiment of half-smooth tongue sole broodstock - the influence on the secretion of sex hormones and other reproductive performance of the broodstock

[0075] 1. Experimental design and experimental feed formula

[0076] Experimental treatment group: implement the different long-chain polyunsaturated fatty acid supply strategies screened in the above experiments at different stages of the maturation-promoting stage of half-smooth tongue sole. During the 3-month maturation-promoting period, the feeding strategy (i.e. the first and second articles in the description of the method of the present invention) that was suitable for the immature stage screened in the above-mentioned experiment was fed in the previous one and a half months. Feed the feeding strategy suitable for the developmental maturity stage screened in the above experiments (ie, the third and fourth items i...

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Abstract

The invention relates to a nutrition method for regulating sex hormone secretion of Cynoglossus semilaevis parent fishes, and belongs to the field of aquatic nutrition. The method is characterized in that different long-chain polyunsaturated fatty acid nutrients are supplied to the Cynoglossus semilaevis parent fishes at different gonad development stages and the parent fishes of different sexes. According to the method provided by the invention, the secretion of sex steroid hormones of the Cynoglossus semilaevis parent fishes can be precisely regulated at different stages for different sexes, the secretion of sex hormones can be promoted, and the breeding performance can be improved; the technology is based on the allocation of essential nutrients in Cynoglossus semilaevis parent fish feeds and high in operability; and the cost is in a controllable range, thereby achieving high economy.

Description

technical field [0001] The invention belongs to the field of aquatic nutrition, and in particular relates to a nutritional method for regulating the sex hormone secretion of Cynoglossus semilaevis broodstock. Background technique [0002] The half-smooth tongue sole is a large warm-temperate bottom fish in the coastal waters of my country. The fish has a docile personality, rapid growth, and fresh and tender meat. It is an important high-value farmed economic fish in northern my country. Since the successful large-scale artificial breeding of tongue sole in 2002, the breeding volume of tongue sole has increased year by year. However, in recent years, the reproductive performance of half-smooth tongue sole broodstock has gradually declined. The gradual reduction of wild tongue sole resources, the increase of breeding generations and unreasonable feeding strategies are the main reasons for the decline in the reproductive performance of half smooth tongue sole broodstock. Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/10A23K50/80A23K20/158
CPCA01K61/10A23K20/158A23K50/80Y02A40/81
Inventor 徐后国梁萌青卫育良曹林
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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