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Stem cell preparation as well as preparation method and application thereof

A stem cell preparation and quality stem cell technology, applied in the field of stem cell preparations and their preparation, can solve the problems of lack of vascular bypass and interventional treatment, lack of distal arterial outflow tract in patients, vascular lesions involving small arteries, etc., and achieve process optimization. , promote wound healing, easy to obtain effect

Inactive Publication Date: 2017-09-29
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The clinical methods used to treat diabetic foot include drug therapy, vascular reconstruction and interventional surgery. However, diabetic patients are old and frail, often suffer from cardiovascular and cerebrovascular diseases, cannot tolerate stimulation such as surgical bypass, and vascular diseases often involve Small arteries, some patients lack distal arterial outflow tracts, and do not have the conditions for vascular bypass and interventional therapy

Method used

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  • Stem cell preparation as well as preparation method and application thereof
  • Stem cell preparation as well as preparation method and application thereof

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preparation example Construction

[0016] The invention provides a stem cell preparation and its preparation method and application, which help to reduce the amputation rate and improve the quality of life of patients. The stem cell preparation provided by the invention includes: fat mesenchymal stem cells, platelet-rich plasma, epidermal growth factor and solvent.

[0017] Further, the cell density of the adipose-derived mesenchymal stem cells is 1×10 5 ~10×10 5 individual / mL.

[0018] Further, the volume percent concentration of the platelet-rich plasma is 5%.

[0019] Further, the working concentration of the epidermal growth factor is 5 ng / mL.

[0020] Further, the adipose-derived mesenchymal stem cells are third to fifth generation adipose-derived stem cells.

[0021] The present invention has no special restrictions on the sources of adipose-derived mesenchymal stem cells, platelet-rich plasma and epidermal growth factor, and those familiar to those skilled in the art can be used, such as commercially...

Embodiment 1

[0031] 1. Formula

[0032] The third generation adipose-derived mesenchymal stem cells 1×10 5 a / mL;

[0033] Platelet-rich plasma (PRP) 5vol%;

[0034] Epidermal growth factor (EGF) 5ng / mL;

[0035] Vehicle make up.

[0036] 2. Preparation

[0037] (1) Collect P3 generation adipose-derived mesenchymal stem cells, absorb the culture medium, add digestion solution for digestion, then stop the digestion with an appropriate amount of serum, gently pipette into a single-cell suspension, then centrifuge at 1500rpm for 5min, discard the supernatant, Wash the cells 3 times with 4°C PBS, resuspend and mix well, adjust the cell density to 10 7 individual / mL.

[0038] (2) Prepare PRP with the PRP preparation kit.

[0039] (3) In a 50mL centrifuge tube, add PRP, EGF, solvent and the P3 generation adipose-derived mesenchymal stem cells prepared in step (1) successively according to the formula ratio, and stir well.

Embodiment 2

[0041] 1. Formula

[0042] Fourth-generation adipose-derived mesenchymal stem cells 5×10 5 a / mL;

[0043] Platelet-rich plasma (PRP) 5vol%;

[0044] Epidermal growth factor (EGF) 5ng / mL;

[0045] Vehicle make up.

[0046] 2. Preparation

[0047] (1) Collect P3 generation adipose-derived mesenchymal stem cells, absorb the culture medium, add digestion solution for digestion, then stop the digestion with an appropriate amount of serum, gently pipette into a single-cell suspension, then centrifuge at 1500rpm for 5min, discard the supernatant, Wash the cells 3 times with 4°C PBS, resuspend and mix well, adjust the cell density to 10 7 individual / mL.

[0048] (2) Prepare PRP with the PRP preparation kit.

[0049] (3) In a 50mL centrifuge tube, add PRP, EGF, solvent and the P3 generation adipose-derived mesenchymal stem cells prepared in step (1) successively according to the formula ratio, and stir well.

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Abstract

The invention belongs to the technical field of stem cells and in particular relates to a stem cell preparation as well as a preparation method and application thereof. The stem cell preparation provided by the invention comprises adipose mesenchymal stem cells, platelet-rich plasma and epidermal growth factors. The components are reasonably matched and have synergistic interaction, and wound healing of diabetic feet is promoted comprehensively; as the adipose mesenchymal stem cells which are simple to collect, easy to obtain, low in immunogenicity and free of ethical issue are adopted as seed cells, great significance is achieved for diabetics; and the preparation method provided by the invention is simple in step operation and optimal in process, and large-scale production can be achieved.

Description

technical field [0001] The invention belongs to the technical field of stem cells, and in particular relates to a stem cell preparation and its preparation method and application. Background technique [0002] With social progress and changes in people's lifestyles and diets, the incidence of diabetes is increasing year by year. According to a recent epidemiological study in China, the prevalence of diabetes in China has reached 9.7%, which is higher than the world average of 6.4%. Diabetic foot is one of the serious chronic complications leading to disability and even death in diabetic patients. With the development of population aging in our country, the incidence of diabetic foot is increasing year by year. Improper treatment may lead to amputation or death, and 85% of amputations are caused by ulcers. The occurrence of diabetic foot ulcers reduces the quality of life of diabetic patients, prolongs the hospitalization time, and brings a huge economic burden to society a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/18A61P17/02A61P3/10A61K35/16A61K35/28
CPCA61K35/28A61K9/0019A61K35/16A61K38/1808A61K2300/00
Inventor 陈海佳葛啸虎王一飞赵萌萌王小燕
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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