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Antigen peptide T790M-1 and application to preparation of medicines for treating non-small cell lung cancer thereof

A non-small cell lung cancer, T790M-1 technology, applied in the field of biomedicine

Inactive Publication Date: 2017-09-22
江苏迈健生物科技发展股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some research reports indicate that immunotherapy targeting mutated antigens is feasible, so far only a small number of mutated antigens have been identified as potential targets for immunotherapy

Method used

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  • Antigen peptide T790M-1 and application to preparation of medicines for treating non-small cell lung cancer thereof
  • Antigen peptide T790M-1 and application to preparation of medicines for treating non-small cell lung cancer thereof
  • Antigen peptide T790M-1 and application to preparation of medicines for treating non-small cell lung cancer thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Prediction and Synthesis of Antigenic Peptide T790M-1

[0019] A recent analysis of motif methods showed that the SYFPEITHI supermotif method is an effective prediction system for HLA-A2 binding peptides. Therefore used to predict the 9-mer HLA-A2 binding peptide from EGFR-T790M. Using SYFPEITHI to remotely predict the HLA-A*02:01 restricted CTL epitope of EGF-T790M, a nonapeptide (STVQLIMQL) with a high score (21 points) was obtained.

[0020] The aforementioned antigenic peptides were prepared by chemical synthesis, that is, a very mature solid-phase peptide synthesis method well known in the art, and either the Boc method or the Fmoc method could be used. The specific method is to couple the protected amino acids to the inert solid phase carrier one by one, and then use strong acid to cleave the peptide chain from the carrier, and remove the side chain protection at the same time. The peptide whose amino acid sequence is STVQLIMQL was prepared.

Embodiment 2

[0022] 1. Peripheral blood mononuclear cell isolation experiment

[0023] Under sterile conditions, take 20 mL of fasting venous blood from HLA-A*02:01 positive normal healthy donors, use lymphocyte separation medium to separate mononuclear cells, and prepare cell suspension with a final mass concentration of 2×10 9 / L in a culture flask at 37°C, 5% CO 2 After incubating in the incubator for 2 hours, the non-adherent cells were washed away; the adherent cells (monocytes) were collected for later use.

[0024] 2. Mature DC induction and antigen loading

[0025] Add human granulocyte-macrophage colony-stimulating factor (rhGM-CsF) 1000 mg / L and recombinant human interleukin-4 (rhIL-4) 500 mg / L to the above-mentioned monocytes according to the final mass concentration. 2 concentrations of cytokines, culture until the 5th day by adding (group A) antigen peptide, (group B) only add medium as a control group, at 37 ° C, 5% CO 2 Cultivate under the condition for 2 days, replace th...

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PUM

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Abstract

The invention provides antigen peptide T790M-1 and application to preparation of medicines for treating non-small cell lung cancer thereof. The amino acid sequence of the antigen peptide T790M-1 is shown as SEQ ID NO. 1. The antigen peptide has a function of activating T cells to kill non-small cell lung cancer cells in targeting and provides a novel and promising immunotherapy method for non-small cell lung cancer patients (EGFR-TKIs therapy) in preventing and / or treating EGFR T790M mutations.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to antigenic peptide T790M-1 and its application in the preparation of medicines for treating non-small cell lung cancer. Background technique [0002] Lung cancer is the leading cause of cancer death worldwide. Non-small cell lung cancer accounts for 80% of lung cancer cases. Although the current treatment methods have been greatly developed, the prediction of lung cancer patients is still insufficient. Recently, however, important advances have been made in the treatment of this disease. In particular, epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs), such as gefitinib and erlotinib, have been developed as new treatments for specific non-small cell lung cancers, and these patients Mutations in the tyrosine kinase region of the EGFR gene. Preclinical studies using EGFR-TKI drugs on patients with EGFR-mutated non-small cell lung cancer (such...

Claims

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Application Information

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IPC IPC(8): C07K14/71A61K39/00A61P35/00
CPCA61K39/0011A61K2039/5154C07K14/71
Inventor 张超冷晓燕常菁曹巍虞强
Owner 江苏迈健生物科技发展股份有限公司
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