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A kind of receptor protein specifically combined with mycoplasma genitalium mgpa and its separation method and application

A technology of mycoplasma genitalium and receptor protein, which is applied in the fields of bioengineering and disease diagnosis and prevention, and can solve problems such as invasion of host epithelial cells and adhesion

Active Publication Date: 2020-10-09
NANHUA UNIV
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Problems solved by technology

However, so far, there has been no relevant report about the binding of Mg to the receptor molecules on the host cell membrane through MgPa, which leads to its adhesion or invasion into the host epithelial cells

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  • A kind of receptor protein specifically combined with mycoplasma genitalium mgpa and its separation method and application
  • A kind of receptor protein specifically combined with mycoplasma genitalium mgpa and its separation method and application
  • A kind of receptor protein specifically combined with mycoplasma genitalium mgpa and its separation method and application

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Embodiment Construction

[0033] At present, mass spectrometry technology has played an important role in protein and proteomics research. It can separate different proteins by using the difference of the charge-to-mass ratio (M / Z) of protein molecules, so as to analyze and identify unknown proteins. In the patent of the present invention, the mass spectrometry analysis of about 17kDa protein shows that the 17kDa receptor protein has a strong correlation with CypA protein.

[0034] Obtaining receptor protein and receptor protein antibody helps to study the characteristics and functions of the receptor. In the present invention, CypA protein and CypA antibody are further used to study the binding specificity of rMgPa and receptor protein by ELISA and Far-western blotting methods. The results show that rMgPa can specifically bind to CypA; the specific protein in the target band of about 17kDa can also bind to rMgPa, indicating that the protein of about 17KDa contains CypA protein. The invention utilizes...

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Abstract

The present invention provides a receptor protein capable of specifically binding to Mycoplasma genitalium MgPa, a separation method and uses thereof. According to the present invention, the receptor protein cyclophilin located on the surface of human urethral epithelial cell membrane is screened, wherein the protein is separated from the cell membrane protection of human urethral epithelial cells, has the nucleotide sequence represented by SEQ ID NO.1, and comprises 165 amino acids; the receptor of rMgPa on the human urethral epithelial cell membrane is screened with the improved VOPBA method, such that the pre-experimental basis is provided for the further clarification of the possible adhesion and pathogenesis of Mg, the further design of the receptor mimetic molecule and the antagonist molecule for blocking the adhesion and invasion into host cells of Mg, and Mg infection prevention and treatment; and the receptor protein CypA can be used for preparing the target drug for the treatment or prevention of Mycoplasma genitalium infection diseases.

Description

technical field [0001] The invention relates to a receptor protein specifically binding to Mycoplasma genitalium MgPa—cyclophilin A (CypA), and also provides a separation method and application of the protein, belonging to the technical fields of bioengineering and disease diagnosis and prevention. Background technique [0002] Mycoplasma genitalium (Mg) is a genitourinary mycoplasma isolated from the urethral secretion of a patient with nongonococcal urethritis for the first time. Clinical studies have shown that Mg infection in women can cause cervicitis, pelvic inflammatory disease, and infertility; while infection in men can cause acute and chronic nongonococcal urethritis and infertility; in addition, Mg infection is also associated with human immunodeficiency virus (HIV) Opportunistic infection is closely related and is a synergistic factor of HIV, so it is called AIDS-associated mycoplasma. [0003] The combination of the pathogen and the corresponding receptor prote...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/705C12N9/90G01N33/573A61K38/17A61P15/00A61P31/04
CPCA61K38/00C07K14/705C12N9/90C12Y502/01008G01N33/573G01N2333/99
Inventor 曾燚华邓湘赢王丽
Owner NANHUA UNIV
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