Preparation method for deer blood peptide and anti-fatigue effect of deer blood peptide

Abstract

The invention relates to a preparation method of deer blood peptide and its anti-fatigue effect, belonging to the field of medicine. Drying of deer blood: use a spray dryer, adjust the inlet temperature to 180°C, the outlet temperature to 80°C, and the speed to 26,000-30,000 rpm; deer blood protein hydrolysis: the concentration of deer blood protein is 3%, and alkaline protease is used for hydrolysis , The hydrolysis conditions are: temperature 53°C, time 5.5h, pH 9.5, enzyme amount 1272u / g; purification of deer blood peptide: membrane filtration and gel chromatography. In the present invention, the anti-fatigue experiment of mice is used to study the activity of the deer blood peptide. Animal experiments show that the polypeptide produced by the optimal hydrolysis process can significantly prolong the swimming time and low-pressure hypoxia tolerance of mice, the content of liver glycogen is significantly higher than that of the control group, and the content of blood lactic acid is significantly lower than that of the control group; It has good anti-fatigue effect.

Description

technical field [0001] The invention belongs to the field of medicine. Background technique [0002] Deer blood is the blood in the chest or antler blood of deer family animals. It is hot in nature, sweet and salty in taste, and returns to the liver and kidney meridians. , is a very valuable traditional Chinese medicine. Since ancient times, it has been a good medicine for the dignitaries and the royal family to treat diseases and keep fit, and is deeply favored by relatives. Deer blood is also widely used among the people to treat rheumatism and rheumatoid diseases. Modern animal experiments and clinical studies have confirmed that in addition to the above effects, deer blood is also more effective in treating palpitations, insomnia, forgetfulness, falls, and anti-aging. At the same time, deer blood also has a position that cannot be underestimated in clinical medicine of traditional Chinese medicine. [0003] The water content percentage of deer blood is 80%-81%, the o...

AI Technical Summary

Problems solved by technology

The liquid-phase synthesis method is mainly used to synthesize small molecular peptides, and it is also commonly used in the synthesis of concentrated peptide fragments. The solid-phase synthesis method is superior to the liquid-phase synthesis method in some aspects. peptide chain, and its automated production process is relatively simple. However, due to the limitations of the relevant operating process and experimental supplies in large-scale production, up to now, the solid-phase synthesis method has room for the rapid establishment of peptide libraries.

The disadvantages of the chemical synthesis method are: especially when using the solid-phase synthesis method, we need to take relevant measures to protect some genes on the side chain of the peptide; the linking reagent required in the synthesis process needs to be excessive, and the amount of the acyl carrier is also large; the linking reagent have certain toxicity

[0049] The preparation of active peptides by direct extraction and separation method is to directly extract various native peptides in natural organisms. The extraction process requires the participation and cooperation of various separation and purification technologies to a large extent. The content in the body is not high, so to purify it from the organism to obtain a relatively high-purity monomer compound, a relatively complicated extraction process cannot be avoided. At the same time, its refining process is also very complicated, which will undoubtedly greatly increase Increase the cost of experiments

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