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Yarrowia lipolytica and applications thereof

A Yarrow lipolytic yeast, ventilation fermentation technology, applied in the direction of fermentation, microorganisms, fungi, etc., can solve the problems of cumbersome steps, inability to assimilate and utilize xylose, etc., and achieve the effect of simplified process operation and high conversion rate

Active Publication Date: 2017-09-15
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although its mass conversion rate has been greatly improved compared with the previous technology, reaching a maximum of 63.2%, it needs to be realized under the condition of feeding or continuous fermentation; and it is fermented to the cell density OD 600 When the value is 20 or above, it is necessary to use acid to adjust the pH value to 3.1-4.0, and the steps are cumbersome
In addition, Yarrow lipolytic yeast mentioned in CN201310282059.X cannot assimilate and utilize xylose

Method used

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  • Yarrowia lipolytica and applications thereof
  • Yarrowia lipolytica and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The natural breeding of embodiment 1 Yarrow lipolytica (CCTCC NO:M 2017027)

[0024] Three soil samples were collected from the poplar forest in Weishan Lake Wetland Honghe Tourist Scenic Area, Tengzhou City, Shandong Province, respectively labeled TW-1, TW-2, and TW-3. Then take 1 g each, respectively add to three 1 L Erlenmeyer flasks containing 100 ml of 350 g / l glucose for enrichment and culture for 3 days. and then diluted to 10 -8 Coating was performed on the screening plate medium I, and a total of 3978 single colonies were obtained. The obtained single colonies were spread on the screening plate medium II respectively, and a total of 27 bacterial strains that can grow with D-xylose as a carbon source were obtained.

[0025] Fermentation and breeding were carried out with fermentation medium I and fermentation medium II respectively. After 3 days of fermentation, 1ml of fermentation broth was taken, centrifuged at 5000rpm for 5min, the supernatant was diluted t...

Embodiment 2

[0026] Example 2 Yeast Yarrow lipolytica (CCTCC NO: M 2017027) fermentation production of erythritol

[0027] The starting strain Yarrowia Lipolytica BBE-17 stored in a glycerol tube at -80°C was activated in a 150ml Erlenmeyer flask with a liquid volume of 25ml and an inoculation volume of 2% (V / V). The basic composition is: glucose 100g / l, yeast powder 10g / l. After activation and cultivation for 24 hours, it was inserted into a 1 L baffled Erlenmeyer flask with a fermentation medium, the filling volume was 100 ml, the inoculum volume was 4% (V / V), and the rotation speed was 220 rpm. The composition of the fermentation medium is: glucose 120g / l, yeast powder 10g / l, ammonium citrate 5g / l, magnesium sulfate 0.5g / l, potassium dihydrogen phosphate 0.25g / l. After 48h of fermentation, the erythritol content measured by HPLC was 82.47g / l, figure 1 The middle peak time of 11.643min corresponds to erythritol, and the mass conversion rate of erythritol to glucose is 69.35%.

Embodiment 3

[0028] Example 3 Yarrow lipolytica (CCTCC NO:M 2017027) fermented xylitol

[0029]The starting strain Yarrowia Lipolytica BBE-17 stored in a glycerol tube at -80°C was activated in a 150ml Erlenmeyer flask with a liquid volume of 25ml and an inoculation volume of 2% (V / V). The basic composition is: glucose 100g / l, yeast powder 10g / l. After activating and culturing for 24 hours, it was inserted into a 500ml common Erlenmeyer flask equipped with a fermentation medium, the filling volume was 50ml, the inoculum volume was 2% (V / V), and the rotating speed was 180rpm. The composition of the fermentation medium is: D-xylose mother liquor (calculated as solid matter) 150g / l, yeast powder 10g / l, ammonium citrate 5g / l, magnesium sulfate 0.5g / l, potassium dihydrogen phosphate 0.25g / l. When fermented for 216 hours, the content of xylitol determined by HPLC was 39.50 g / l, and the mass conversion rate of xylitol to D-xylose was 33.35%.

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Abstract

The invention discloses a Yarrowia lipolytica and applications thereof, and belongs to the field of industrial biotechnology. A strain obtained after separation is named as Yarrowia lipolytica BBE-17. The strain can produce erythritol through fermentation using glucose as a carbon source, a high conversion rate can be realized without fed batch or continuous fermentation, and the adjustment of the pH value is not needed in the whole fermentation process, so the technologic operation is simplified, and the strain has certain industrial application potential. The strain also can produce xylitol through fermentation using a D-xylose mother liquor as a carbon source, so the strain a new potential way for the preparation of polyol products from non-food raw materials through a biological technology.

Description

technical field [0001] The invention relates to a Yarrow lipolytic yeast strain and application thereof, belonging to the field of industrial biotechnology. Background technique [0002] Yarrow lipolytic yeast (English: Yarrowia Lipolytia and other Chinese names: Yarrowia lipolytica, Yarrowia lipolytic yeast, Yarrowia lipolytica, etc.) has a wide range of applications in the field of biomanufacturing. For example: CN03817060.4 discloses a technology for producing citric acid using Yarrow lipolytica; CN200910199533.6 and CN201511019785.8 disclose a technology for producing gamma-decalactone using Yarrow lipolytica; CN201210463413.4 And CN201310282059.X discloses a kind of technology that uses Yarrow lipolytica to produce erythritol; CN201610304562.4 discloses a kind of technology that utilizes Yarrow lipolytica to produce epoxy oleic acid; CN201010578594.6 and CN201310481832.5 A technique for producing α-ketoglutarate by using Yarrow lipolytica is disclosed; CN201410243227.9...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P7/18C12R1/645
CPCC12P7/18C12N1/145C12R2001/645
Inventor 李江华张娟陈坚邱学良堵国成朱政明
Owner JIANGNAN UNIV
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