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Colorectal cancer stem cell vaccine preparation method and application

A technology of colorectal cancer and stem cells, which is applied in the field of preparation of colorectal cancer stem cell vaccines to achieve good anti-tumor effects, enhance killing activity and CDC activity, and increase IFN-γ levels

Inactive Publication Date: 2017-09-15
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the differences between the human immune system and mice, the immune effect of colorectal cancer stem cell vaccine on the human body needs further clinical verification

Method used

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  • Colorectal cancer stem cell vaccine preparation method and application
  • Colorectal cancer stem cell vaccine preparation method and application
  • Colorectal cancer stem cell vaccine preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Preparation of colon cancer cells:

[0041] Step 1, after aseptically obtaining the tumor tissue, wash the tumor tissue with PBS containing double antibodies (100U / mL penicillin and 100U / mL streptomycin), remove necrotic tissue, connective tissue, blood vessels, fat, etc., and cut the tumor tissue with scissors Shredded into 0.5 ~ 1mm 3 Add collagenase and DNase to the tissue block, digest and enzymatically hydrolyze, filter, and prepare single-cell suspension;

[0042] Step 2, put the cell suspension in a culture bottle, after the cells adhere to the wall, remove the suspended cells, replace with fresh culture medium, and subculture.

[0043] Preparation of colorectal cancer stem cell vaccine:

[0044] Step 1. Obtain and subculture tumor tissue cells after colorectal cancer surgery or human colorectal cancer cell line Lovo cells or mouse colorectal cancer cell line CT26 cells, using RPMI1640 containing 10% calf serum as the medium, 37°C , 5%CO 2 Culture under satur...

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Abstract

The invention discloses a colorectal cancer stem cell vaccine preparation method and application. Postoperative tumor tissue cells derived from patients with colorectal cancer, human colorectal cancer cell lines Lovo or mouse colorectal cancer cell lines CT26 are prepared into CD133+ colorectal cancer stem cell vaccines by a repeated freezing-thawing method. The CD133+ colorectal cancer stem cell vaccines can reduce immunized mouse serum TGF-beta level, IFN-gamma level is improved, killing activity of NK (natural killer) cells and CDC (complement-dependent cytotoxicity) activity are enhanced, immunized mice resist colorectal cancer cell attack, immune cells are induced to selectively kill CD133+ colorectal cancer stem cells, and further tumor growth is inhibited.

Description

technical field [0001] The invention belongs to the technical field of biological products, and in particular relates to a preparation method and application of a colorectal cancer stem cell vaccine. Background technique [0002] Colorectal cancer (Colorectal Cancer) is one of the common malignant tumors of the digestive system, ranking second in the cause of cancer-related deaths. Compared with developing countries in the Asia-Pacific region, colorectal cancer is more likely to occur in developed countries such as Europe and the United States. According to the 2017 China City Cancer Report: Colorectal cancer ranks fifth in male incidence and third in female incidence. About 50% of colon cancer patients experience a cancer recurrence in the first three years after surgery, and the five-year survival rate depends on cancer. Metastasis to distant organs decreased significantly. Studies suggest that during the evolution of colorectal cancer, gene mutations and changes in the ...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61P35/00C12N5/09
CPCA61K39/0011A61K2039/5152C12N5/0693C12N2509/00
Inventor 窦骏郭玫赵枫姝王净侍方方
Owner SOUTHEAST UNIV
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