Method for producing animal anti-human serum by utilizing immune time variation

Abstract

The invention discloses a method for producing animal anti-human serum by utilizing immune time variation. The method comprises the following steps: preparing immunogen emulsion, performing repeated immune injection, sampling blood, separating serum and detecting double-expanding valence thereof, wherein the time interval of the repeated immune injection is controlled in the manner of arithmetic progression decreasing. The invention overcomes the defects of multiple unclear immune expressions in all the original text books and data reports; the method is accurate and is easily learned, mastered and controlled; the immune period is shortened and the production cost is saved; all the immune responses of the body can be fully called, so that the antigen which is difficult to be immunized also can generate excellent antibody valence; individual difference between the goats can be overcome, the antibody valence is uniform and a stable raw material can be supplied for IVD industry; the application of the biotechnology to the scale production at the aspect of animal antibody preparation becomes possible; excellent antibody valence is generated after six times of immunization of the universal antigen, so that the time of one immunization period is reduced by a half or above as compared with the original immunization time.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a method for producing animal anti-human serum by utilizing the variation of immune time. Background technique [0002] Antiserum is serum containing a certain type of antibody molecule with specific immune function, generally animal serum prepared after animals are artificially injected with a certain type of antigen. Whether an antigen can cause antibody production depends on the one hand whether there is an antigenic determinant on the surface of the antigen molecule, and on the other hand depends on the immune status of the body. When the above two conditions are met, antibody production will follow the antibody General law of generation - first reaction and second reaction. There are many kinds of antigens, including natural protein antigens and cellular antigens, synthetic antigens and genetic engineering antigens, etc. Different antigens have different specificit...

AI Technical Summary

Problems solved by technology

[0004] At present, when producing sheep anti-human serum, the time interval between each immunization injection is long, which leads to a long production cycle and high cost, and there are individual differences among sheep, so that the antibody titer is not uniform

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