Method applicable to rapid propagation of various plants
A plant and fast technology, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of increasing manpower and material resources, different tissue culture and reproduction methods, affecting factory production costs, etc., to reduce production costs , Reduce the effect of manpower and material resources
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Embodiment 1
[0027] Example 1, Tissue Culture Rapid Propagation of Honeysuckle, Houttuynia cordata, Yaerqin, Shuangjiulixiang
[0028] 1. Culture medium preparation and sterilization
[0029] Primary medium: ER+6-BA1.2mg / L+KT0.5mg / L+3% sucrose+0.75% agar.
[0030] Proliferation medium: ER+KT2.0mg / L+paclobutrazol1.0mg / L+3% sucrose+0.75% agar. The above medium was sterilized at 121°C for 20 minutes.
[0031] 2. Primary culture
[0032]In step (1) of the above method, the stem section of the annual branch of honeysuckle is selected as the explant for cultivation, and the stem section is cut into 1.0-2.0 cm for later use; after the rhizome of Houttuynia cordata is first cleaned, it is placed in clean water and cultivated for about 10 days, and then the Cut the rhizomes into 1.0-2.0 cm and set aside; select smaller plants for duck celery, remove the roots, petioles and leaves, keep the base at a height of about 2 cm, and peel off several layers of bracts for later use; peel off four numbers ...
Embodiment 2
[0038] Example 2, Tissue Culture Rapid Propagation of Honeysuckle, Houttuynia cordata, Yaerqian, Shuangjiulixiang
[0039] 1. Culture medium preparation and sterilization
[0040] Primary medium: ER+6-BA1.5mg / L+KT0.5mg / L+3% sucrose+0.75% agar.
[0041] Proliferation medium: ER+KT2.5mg / L+paclobutrazol 1.5mg / L+3% sucrose+0.75% agar. The above medium was sterilized at 121°C for 20 minutes.
[0042] Different explants of honeysuckle, Houttuynia cordata, Yaerqin, and Sishu Jiulixiang were taken from tissue culture to rapidly propagate different explants, and the basic operation method was the same as that of Example 1; Partialized more brown matter, but does not affect its growth.
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