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Detection marker for diabetic nephropathy

A technology of diabetic nephropathy and markers, applied in the field of diabetic nephropathy detection markers, can solve problems such as difficult early detection of diabetic nephropathy, and achieve the effects of convenient collection, good specificity, and improved specificity and accuracy

Inactive Publication Date: 2017-09-08
LANZHOU BAIYUAN GENE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Diabetic nephropathy is a common complication of diabetes. It is one of the manifestations of systemic microvascular disease in diabetes. The clinical features are proteinuria, progressive renal damage, hypertension, edema, and severe renal failure in the late stage. It is the main cause of death in diabetic patients. One, it is difficult to detect diabetic nephropathy early by conventional detection methods

Method used

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  • Detection marker for diabetic nephropathy
  • Detection marker for diabetic nephropathy
  • Detection marker for diabetic nephropathy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Diabetic nephropathy detection marker of the present invention is:

[0030] Keratin 19, haptoglobin, apolipoprotein E, C-reactive protein, platelet factor 4 precursor, sex hormone-binding globulin precursor, immunoglobulin heavy chain Viiiregionhil, complement component C4, keratin 6l, C-reactive protein precursor Splice isoform 1, serine / threonine protein kinase WNK4 and CD 5 antigen.

Embodiment 2

[0032] In this application, 12 kinds of proteins in serum or urine (ie, keratin 19, haptoglobin, apolipoprotein E, C-reactive protein, platelet factor 4 precursor, sex hormone-binding globulin precursor, Immunoglobulin heavy chain Viiiregionhil, complement component C4, keratin 6l, C-reactive protein precursor splice isoform 1, serine / threonine protein kinase WNK4 and CD 5 antigen), and then buckle the differential spots on the two-dimensional electrophoresis gel Points, do mass spectrometry, determine the name of the protein and its size, and compare and analyze the protein content in the serum or urine of the healthy control group and diabetic nephropathy patients. The specific operation is as follows:

[0033] 1. The detection substance is serum

[0034] 1. Prepare healthy control serum: The healthy control serum is collected from healthy people, and the expression levels of the above 12 proteins in the serum are within the normal range.

[0035] The healthy control serum...

Embodiment 3

[0137] Diabetic nephropathy detection kit of the present invention:

[0138] 1. Collect serum from 2-3 healthy people, and the expression levels of the above 12 proteins in the serum are within the normal range. Freeze at -80°C after collection. as a standard negative serum;

[0139] 2. Prepare the solution used for electrophoresis (if there is any improvement, just write it here, corresponding to Example 2);

[0140] 3. Prepare the standard plate: put figure 1 with Image 6 Printed, as a standard plate. After the detection, it can be judged according to the electrophoresis results of the standard plate or the standard negative serum.

[0141] The detection method of the detection kit of the present invention is carried out according to Example 2 (the step of performing mass spectrometry analysis on the difference points is omitted).

[0142] The test results are judged based on: when keratin 19, haptoglobin, apolipoprotein E, C-reactive protein, platelet factor 4 precur...

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Abstract

The invention provides a detection marker for diabetic nephropathy. The detection marker for the diabetic nephropathy consists of 12 proteins: keratin 19, haptoglobin, apolipoprotein E, C-reactive protein, a platelet factor 4 precursor, a sex hormone binding globulin precursor, an immunoglobulin heavy chain Viiiregionhil, a complement component C4, keratin6l, a C-reactive protein precursor splicing isoform 1, serine / threonine protein kinase WNK4 and a CD 5 antigen. Compared with an existing detection method, the detection marker provided by the invention has the advantages of high specificity, sensitivity and accuracy, and can be used as a basis for diagnosing the diabetic nephropathy.

Description

technical field [0001] The invention relates to a diabetic nephropathy detection marker. Background technique [0002] Diabetic nephropathy is a common complication of diabetes. It is one of the manifestations of systemic microvascular disease in diabetes. The clinical features are proteinuria, progressive renal damage, hypertension, edema, and severe renal failure in the late stage. It is the main cause of death in diabetic patients. One, it is difficult to detect diabetic nephropathy early by conventional detection methods. Contents of the invention [0003] The invention provides a diabetic nephropathy detection marker, which is used to detect diabetic nephropathy and has good specificity, sensitivity and accuracy. [0004] Blood has become one of the best sources of disease markers due to the convenience of sampling and the ability to better reflect the pathophysiological process of the body. However, it is difficult to find protein markers with high specificity and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447
CPCG01N27/44773G01N27/44795
Inventor 车团结常运朝吴玲李琳陈小兰吕海宏
Owner LANZHOU BAIYUAN GENE TECH
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