Heavy water sealing method and method for detecting multi-photon signal strength in multi-photon imaging

Abstract

The invention is applicable to the technical field of biophotonics and provides a heavy water sealing method. The heavy water sealing method comprises the step of dripping paroline to the surface of heavy water to cover the surface of the heavy water. The invention further provides a method for detecting multi-photon signal strength in multi-photon imaging. The method comprises the following steps: dripping the heavy water to a slide provided with a to-be-tested sample, and putting the slide provided with the to-be-tested sample into a multi-photon imaging system; dripping paroline to the surface of the heavy water to cover the surface of the heavy water; irradiating the to-be-tested sample by virtue of laser generated by the multi-photon imaging system so as to generate multi-photon signals, and collecting the multi-photon signals by virtue of a detector in the multi-photon imaging system; and detecting the multi-photon signal strength based on the collected multi-photon signals, so as to judge whether the change of the multi-photon signal strength is in a threshold range. According to the detection method, paroline has a very good effect on sealing the heavy water, so that the strength of the generated multi-photon signals is not attenuated along with time.

Description

technical field [0001] The invention belongs to the technical field of biophotonics, and in particular relates to a method for sealing heavy water and a method for detecting multiphoton signal intensity in multiphoton imaging. Background technique [0002] Multiphoton microscopy is an imaging technique based on nonlinear optical effects, which has a wide range of applications in biomedicine. There are two main research directions in multiphoton imaging. One is to increase the imaging depth without damaging biological tissues, and the other is to expand the imaging modality to obtain more sample information. Research in both directions has benefited from the development of lasers. Recently, some experiments have shown that the high-energy soliton laser in the 1700nm band is used as the excitation light source, and the deep brain tissue of living mice can be imaged by multiphoton imaging technology. Microscopic imaging techniques for imaging depth and extended multiphoton im...

AI Technical Summary

Problems solved by technology

However, this method has several disadvantages: (1) Before replacing the new heavy water, the heavy water contaminated by water vapor must be carefully and completely removed to ensure that there is no residue

However, this method cannot be used for time-kinetic measurements based on fluorescence intensity, because the attenuation of the signal caused by the absorption of water vapor by heavy water will be superimposed on the attenuation curve of the fluorescent dye, causing errors in the experimental results

Images