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Applications of Kindlin-2 protein as target point in preparing medicines for treating nephritis

A protein and drug technology, applied in gene therapy, drug combination, biological testing, etc., can solve the problem of lack of nephritis drugs and treatment methods

Active Publication Date: 2017-09-08
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, there is still a lack of effective drugs and therapeutic methods for the treatment of nephritis clinically at present.

Method used

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  • Applications of Kindlin-2 protein as target point in preparing medicines for treating nephritis
  • Applications of Kindlin-2 protein as target point in preparing medicines for treating nephritis
  • Applications of Kindlin-2 protein as target point in preparing medicines for treating nephritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Effect of Knockout of Kindlin-2 Gene on the Growth Curve of Mice

[0058] The gene knockout technology was used to specifically knock out Kindlin-2 in mouse glomerular podocytes, and the total RNA of the corresponding tissue was extracted after knockout, and then quantitatively analyzed by real-time qPCR. By measuring the total RNA and glyceraldehyde-3 in the corresponding tissue -The ratio of phosphate dehydrogenase (GAPDH) judges whether the gene knockout is specific. The results showed that the knockout of Kindlin-2 in cKO mice was specific and limited to the kidney, and other tissues such as liver, islets, heart, lung, etc. had no significant difference from the normal group. Both the experimental group and the control group began to measure the body weight of the mice one week after birth, and 12 mice in each group were weighed once a week for 8 consecutive weeks, and the results were drawn as follows: figure 1 , animal growth curves showed that cKO mice grew slow...

Embodiment 2

[0061] Effect of Knockout of Kindlin-2 Gene on Urinary Protein and Plasma Protein Content of Mice

[0062] Mouse urine protein test

[0063] The urinary protein in the urine of the mice in the experimental group and the control group was collected respectively, and the same volume was taken for loading and SDS-PAGE protein electrophoresis experiment. The obtained electrophoresis results were as follows: image 3 shown, where image 3 The electrophoresis samples from left to right in the center are as follows, the first lane is Laoder (protein marker), the second lane is BSA sample (bovine serum albumin, as the experimental reference), and the third lane is WT mouse after 1 week The urine protein of the mice in the 4th lane is the urine protein of the cKO mice after 1 week, the urine protein of the WT mice in the 5th lane is the urine protein of the mice of the WT type after 2 weeks, and the 6th lane is the urine protein of the cKO mice after 2 weeks Protein, the 7th lane is ...

Embodiment 3

[0068] Further, ELISA detection kits were used to detect urinary albumin, creatinine and plasma creatinine of mice in the experimental group and control group in different periods, and albumin / creatinine was used as the ordinate. The results are as follows: Figure 5 As shown, with plasma creatinine as the ordinate, the results are as follows Figure 6 shown. From the above results, it can be seen that at different time points, the ratio of urinary albumin to creatinine in cKO mice was significantly increased, and the plasma creatinine content in cKO mice was also significantly increased. The kidneys of the 8-week-old mice in the experimental group and the control group were taken respectively, and the results were as follows: Figure 7 As shown, the kidneys of cKO mice were significantly lighter in color, smaller and irregular in shape.

[0069] The above results further show that after Kindlin-2 gene-specific knockout, the ratio of urinary albumin to creatinine in cKO mice...

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Abstract

The invention relates to applications of Kindlin-2 protein as a target point in preparing medicines for treating nephritis. The research result shows that after the Kindlin-2 gene is specifically knocked out, the content of urine protein of mice is obviously increased, while the content of plasma protein is obviously reduced, meanwhile, the structure of glomeruli of the kidney is severely damaged, and the obvious glomerular sclerosis and interstitial fibrosis exist. The experimental result of the research shows that Kindlin-2 protein can be taken as a novel target point for preparing the medicines for treating nephritis.

Description

technical field [0001] The invention relates to the field of medicines, in particular to the application of a Kindlin-2 protein as a target in the preparation of medicines for treating nephritis. Background technique [0002] Nephritis is a common kidney disease that mainly occurs in bilateral kidney glomeruli, often manifested as edema, proteinuria, hematuria, and decreased renal function. As my country enters into an aging society, the number of patients with kidney disease is increasing at an alarming rate, and the incidence rate has reached as high as 10.8%. Long-term fatigue, environmental pollution, drug abuse and other factors can lead to varying degrees of damage to the kidneys, and even develop chronic kidney disease. Once chronic kidney disease occurs, it will not be easy to cure, and long-term drug treatment may further increase the burden on the kidneys. Therefore, it is very urgent to develop new drugs for the treatment of nephritis. [0003] However, there i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K48/00A61K47/46A61K47/26A61K47/51A61K47/54A61K47/69A61P13/12G01N33/58G01N33/68
CPCA61K45/00A61K47/26A61K47/46A61K48/0025A61K48/005G01N33/58G01N33/6887G01N33/6893G01N2800/347
Inventor 肖国芝曹惠玲
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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