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Method for culturing flax tissue

A flax and tissue culture technology, which is applied in the field of plant propagation and flax tissue culture, can solve the problems of imperfection, limited flax planting quantity, and failure to reach industrialized production, so as to increase production, increase quantity, and reduce cultivation time Effect

Inactive Publication Date: 2017-09-01
CHENGDU MIAOFU MODERN NURSERY STOCK SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Over the years, research on flax has been going on, and many research results have been obtained. However, the research on the regeneration system of specific flax genotypes is still not perfect. The amount of flax planted each year is limited and cannot reach industrial production. requirements

Method used

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Embodiment Construction

[0014] The present invention will be further described below in conjunction with specific examples.

[0015] Flax tissue culture method, comprises the steps:

[0016] Step 1: Take healthy flax seeds, put them into a sterile petri dish, and then put the petri dish into an airtight container filled with chlorine gas, sterilize for 24 hours, and set aside.

[0017] Step 2: inoculate the sterilized seeds into B5 medium for germination culture, and culture for 6 days to obtain germinated flax seedlings.

[0018] Step 3: Cut off the shoot tips of the well-growing aseptic flax seedlings, and inoculate them at a concentration of 1.5 mg·L of 6-benzylaminoadenine -1 and indole-3-acetic acid at a concentration of 0.2 mg·L -1 In MSB medium, cultivated for 30d. Differentiated and multiplied flax seedlings were obtained.

[0019] Step 4: Inoculate the obtained seedlings to 1 / 2B5 and NAA concentration of 0.02 mg·L by subculture -1 15 days on rooting medium. At this time, the seedlings ...

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Abstract

The invention discloses a flax tissue culture method, which comprises the following steps: step 1: take healthy flax seeds, put them into a sterile petri dish, and sterilize them; step 2: inoculate the sterilized seeds into B5 medium for Germination culture is carried out for 6 days to obtain germinated flax seedlings. Step 3: cutting off the shoot tip of the aseptic flax seedling, inoculating it into MSB medium, and culturing it to obtain differentiated and proliferated flax seedlings. Step 4: through subculture, inoculate the obtained seedlings to 1 / 2B5 and a rooting medium with a concentration of 0.02 mg·L-1 of naphthaleneacetic acid and cultivate them for 15 days. Step 5: Move the cultured test tube seedlings indoors for 3-4 days, and then transplant them into cultivation soil for growth. Large-scale soil cultivation can be realized through tissue-cultured flax seedlings. The quantity of flax cultured by the method is greatly increased, and the culture time is also greatly reduced.

Description

technical field [0001] The invention relates to a plant propagation method, in particular to a flax tissue culture method, which belongs to the technical field of plant propagation. Background technique [0002] Flax is an annual herb and the earliest natural plant fiber used by humans. It can be divided into three types: flax for fiber, flax for oil and flax for both oil and fiber. The stems are erect, up to 1.2 meters high, and the upper part is soft and waxy; the leaves are alternate, lanceolate, 20-40 mm long, 3 mm wide, and glaucous on the surface; the flowers have 5 petals, 15-25 in diameter mm, blue or white; the fruit is a capsule, the seed is flat and oval, and it likes cool and humid climate. Flax is a kind of pure natural fiber, because of its outstanding characteristics such as sweat absorption, good air permeability and harmlessness to human body, it has been paid more and more attention by people. At the same time, flax is also an oil crop. Flax oil contains ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 但晓灵
Owner CHENGDU MIAOFU MODERN NURSERY STOCK SCI & TECH
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