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Skin barrier function-improving agent and composition for improving skin barrier function

A skin barrier and improving agent technology, applied in skin care preparations, drug combinations, skin diseases, etc., can solve problems such as insufficient moisture retention function, and achieve the effect of improving moisture retention function

Inactive Publication Date: 2017-08-29
SHOWA DENKO KK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] Conventional skin barrier function improvers have insufficient ability to improve the moisture retention function of the skin

Method used

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  • Skin barrier function-improving agent and composition for improving skin barrier function
  • Skin barrier function-improving agent and composition for improving skin barrier function
  • Skin barrier function-improving agent and composition for improving skin barrier function

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0139] (Expression promotion effect of TRPV4 gene)

[0140] Human skin-derived epidermal cells (SVHK cells) obtained by introducing the SV40 gene into human skin-derived epidermal cells were cultured at 10,000 cells / cm 2 The seeding density was inoculated in a plastic petri dish, and cultured for 24 hours in Dulbecco's modified Eagle medium (DMEM) medium manufactured by Sigma Corporation containing 10% fetal bovine serum.

[0141]Next, an inositol derivative dissolved in purified water (the composition will be described later) was added to the culture medium of the SVHK cells so that the final concentration was 1 μM, and further cultured for 48 hours. As a control, SVHK cells to which the same amount of purified water was added instead of 1 μM of the inositol derivative were cultured in the same manner as above. Also, for comparison, SVHK cells to which 1 μM myo-inositol dissolved in purified water was added instead of the myo-inositol derivative were cultured in the same man...

experiment example 2

[0147] (Expression promotion effect of TJ-related genes)

[0148] Human skin-derived epidermal cells (SVHK cells) obtained by introducing the SV40 gene into human skin-derived epidermal cells were cultured at 10,000 cells / cm 2 The seeding density was inoculated in a plastic petri dish, and cultured for 24 hours in Dulbecco's Modified Eagle (DMEM) medium manufactured by Sigma containing 10% fetal bovine serum.

[0149] Next, 1 μM of an inositol derivative dissolved in purified water (the composition will be described later) was added to the culture medium of the SVHK cells so that the final concentration was 1 μM, and further cultured for 48 hours. As a control, SVHK cells to which the same amount of purified water was added instead of the inositol derivative were cultured in the same manner as above. Also, for comparison, SVHK cells to which 1 μM myo-inositol dissolved in purified water was added instead of the myo-inositol derivative were cultured in the same manner as above...

experiment example 3

[0156] (TJ formation ability evaluation test)

[0157] The skin model (model "EP1-200", manufactured by Kurabo Textile Co., Ltd.) was heated at 37°C and 5% CO 2 24 hours before incubation. Next, inositol derivatives (the composition will be described later) dissolved in purified water at concentrations of 0.1, 0.5 and 1 w / v% were added to the surface of the skin model, and cultured for a further 24 hours.

[0158] Next, the inositol derivative was removed, washed three times with phosphate buffer, and then the transepidermal water evaporation (TEWL) was measured. For the measurement of TEWL, a Cyclon moisture evaporator (manufactured by Asah Biomed Co., Ltd.) was used. The inositol derivative used was analyzed by liquid chromatography-mass spectrometry (LC-MS), and as a result, the ratio of molecules with one glucose bonded to the glucose chain of inositol was 12% by mass % , the proportion of molecules with 2 glucose numbers is 30%, the proportion of molecules with 3 gluco...

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Abstract

The invention relates to a skin barrier function-improving agent that comprising, as an active ingredient, an inositol derivative containing an inositol and a saccharide bonded together, and a composition for improving skin barrier function that comprises the skin barrier function-improving agent and a pharmaceutically acceptable carrier.

Description

technical field [0001] The present invention relates to a skin barrier function improving agent and a composition for improving skin barrier function. this application claims priority based on Japanese Patent Application No. 2014-220711 for which it applied in Japan on October 29, 2014, and takes in the content here. Background technique [0002] Transient receptor potential vanilloid 4 (TRPV4) is a protein discovered as an osmosensitive receptor activated by low osmolarity. [0003] It has been reported that TRPV4 functions as a water evaporation sensor, and is activated by 4α-phorbol ester (4α-PDD) to inhibit water evaporation and improve skin barrier function (for example, refer to Non-Patent Document 1 and Non-Patent Document 2. ). [0004] Tight junctions (TJs) are deeply related to skin barrier function. TJ is a band-shaped intercellular adhesion structure formed around epithelial cells. TJ prevents the entry and exit of substances through the intercellular space b...

Claims

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Application Information

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IPC IPC(8): A61K31/7034A61K8/60A61P17/16A61P43/00A61Q19/00
CPCA61Q19/00A61K8/73A61Q19/08A61K31/7032A61K2800/78A61K8/602A61P17/16A61P43/00A61K31/7034A61Q17/00
Inventor 佐伯夕子加藤咏子
Owner SHOWA DENKO KK
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