Animal selenium-enriched health-care nutritional liquid, preparation method and application
A health-care nutrient solution and selenium-enriched technology, which is applied in applications, animal feed, animal feed, etc., to reduce the occurrence of diseases, improve immunity and resistance, and reduce the harm caused by the environment and human body
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Embodiment 1
[0039] The concrete components of the selenium-enriched animal health care nutrient solution of the present embodiment are as follows:
[0040]
[0041]
[0042] The preparation method of the present embodiment is as follows:
[0043] (1) Breeding of selenium-enriched yeast strains
[0044] Candida utilis was cultured on a YEPD culture plate for 24 hours, and a single colony was picked into a 250ml Erlenmeyer flask containing 100ml YEPD liquid medium, 180rpm, shaking culture for 36 hours, and the bacteria liquid was centrifuged and washed 3 times to make a single cell suspension. solution, so that the cell concentration is 10 8 ~10 9individual / mL. Take 0.1mL of the bacterial suspension and evenly spread it on a sterile petri dish, and place it on a clean bench to air dry to form a bacterial film. in N + Ion implantation is carried out in the implanter with an energy of 18KeV. Six gradient doses were made for each strain, with two plates for each dose. N + The inj...
Embodiment 2
[0065] The specific components of the selenium-enriched animal health care nutrient solution of the present embodiment are as follows:
[0066]
[0067]
[0068] The preparation method of the present embodiment is as follows:
[0069] (1) Breeding of selenium-enriched yeast strains is the same as in Example 1.
[0070] (2) Yeast hydrolyzate obtained
[0071] The above-mentioned Candida utilis cas15 was inoculated in 500 mL of fermentation medium, and cultured with shaking at 28° C. and 170 rpm for 60 h to obtain a selenium-enriched yeast culture. The composition of the fermentation medium is: malt extract 15g / L, peptone 12g / L, yeast extract 8g / L, sodium selenite 150mg / L.
[0072] Collect the selenium-enriched yeast culture, centrifuge at 9000rpm, centrifuge for 10 minutes, wash, add a mixed enzyme preparation of wall-breaking enzyme and papain in a mass ratio of 1:1, and enzymatically hydrolyze for 20 hours to obtain organic selenium and amino acids. of yeast hydrolyz...
Embodiment 3
[0090] The specific components of the selenium-enriched animal health care nutrient solution of the present embodiment are as follows:
[0091]
[0092] The preparation method of the present embodiment is as follows:
[0093] (1) Breeding of selenium-enriched yeast strains is the same as in Example 1.
[0094] (2) Yeast hydrolyzate obtained
[0095] The above-mentioned Candida utilis cas15 was inoculated in 500 mL of fermentation medium, and cultured with shaking at 28° C. and 180 rpm for 72 hours to obtain a selenium-enriched yeast culture. The composition of the fermentation medium is: malt extract 20g / L, peptone 15g / L, yeast extract 10g / L, sodium selenite 180mg / L.
[0096] Collect the selenium-enriched yeast culture, centrifuge in a centrifuge, 10000rpm, centrifuge for 10min, wash, add a mixed enzyme preparation of wall-breaking enzyme and papain in a mass ratio of 1:1, and enzymatically hydrolyze for 24 hours to obtain organic selenium and amino acids of yeast hydrol...
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