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Method for cultivating strong seedlings for vaccinium uliginosum tissue culture and rapid propagation based on color mixing LED light source

A LED light source, tissue culture rapid propagation technology, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problem of reducing the production cost of Vaccinium vaccinium tissue culture, thin and transplanted Vaccinium vaccinia Low planting survival rate and other problems, to save culture medium and labor input, shorten the time for multiplication and rooting and root culture, and improve the multiplication coefficient

Pending Publication Date: 2017-08-29
BEIJING FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0006] The object of the present invention is to provide a method for fast-growing seedlings of Vaccinium vaccinium tissue culture based on color-mixing LED light source, which can effectively solve the problems of thin, weak, poor adaptability, and low survival rate of transplanting Vaccinium vaccinia. It can reduce the cost of tissue culture production of lingonberry

Method used

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  • Method for cultivating strong seedlings for vaccinium uliginosum tissue culture and rapid propagation based on color mixing LED light source
  • Method for cultivating strong seedlings for vaccinium uliginosum tissue culture and rapid propagation based on color mixing LED light source

Examples

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Effect test

Embodiment 1

[0029] Example 1 According to the above-mentioned experimental steps, the red light intensity is set to 300Lx, the blue light intensity is set to 900Lx, and the red-blue light intensity ratio is 1:3.

Embodiment 2

[0030] Example 2 According to the above-mentioned experimental steps, the red light intensity is set to 600Lx, the blue light intensity is 600Lx, and the ratio of red and blue light is 1:1.

Embodiment 3

[0031] Example 3 According to the aforementioned experimental steps, the red light intensity is set to 900Lx, the blue light intensity is set to 300Lx, and the ratio of red and blue light is 3:1.

[0032] The control example follows the test procedure described above, the only difference is that the light conditions of the proliferation culture and the rooting culture are white fluorescent lamps with a light intensity of 1200 Lx.

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Abstract

The invention belongs to the plant tissue culture and rapid propagation field, and mainly relates to a method for cultivating strong seedlings for vaccinium uliginosum tissue culture and rapid propagation based on a color mixing LED light source. According to the method, red and blue color mixing LED light sources which are in a certain proportion are used for conducting strong seedling treatment respectively in a vaccinium uliginosum seedling multiplication phase and a rooting phase. By adopting the method, the propagation coefficient of vaccinium uliginosum is improved; the rooting cycle is shortened; the growth of stems, leaves and root systems is accelerated; vaccinium uliginosum tissue culture seedlings grow thick and strong; leaf areas are enlarged; and the transplanted survival rate is improved. According to the vaccinium uliginosum tissue culture seedlings cultivated by the method, the content of soluble sugar and anthocyanin in the leaves is obviously improved; the anti-adversity ability of the seedlings can be effectively improved; and the strong seedling transplantation and the subsequent adaptive capacity of the seedlings are improved by a large margin. Compared with used traditional white fluorescent lamps, the red and blue color mixing LED light sources save electric energy effectively; the cost is reduced while the quality of the vaccinium uliginosum tissue culture seedlings is comprehensively improved; and an efficient and economical method for cultivating the strong seedlings for the vaccinium uliginosum tissue culture and rapid propagation is provided.

Description

Technical field: [0001] The invention relates to the technical field of plant tissue culture and rapid propagation, in particular to a method for tissue culture, rapid propagation and strong seedlings of Vaccinium cranberry based on a mixed-color LED light source. Background technique: [0002] Vaccinium uligiosum Linn. is a deciduous shrub of the Rhododendronaceae Vaccinium genus. It is extremely cold-resistant. It is concentrated in the Greater and Lesser Khingan Mountains and Changbai Mountains in China. It is one of the wild berry resources with great development and utilization value. It is commonly known as wild blueberry. . Its fruit is blue-purple, rich in flavonoids, polyphenols, anthocyanins, proanthocyanidins and other active substances. It has significant effects on preventing cancer, cardiovascular, anti-aging, improving eyesight, and improving immunity. It is recognized by the International Food and Agriculture Organization Listed as one of the five major heal...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 侯智霞万如萌陈露
Owner BEIJING FORESTRY UNIVERSITY
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