Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Screening method of molecular marker of circadian rhythm behavior related gene Cry1 of egg ducks

A circadian rhythm, molecular marker technology, applied in biochemical equipment and methods, DNA/RNA fragments, DNA preparation, etc., to achieve the effect of eliminating abnormal behavior

Inactive Publication Date: 2017-08-22
GUIZHOU UNIV
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, there have been a large number of research reports on molecular markers and their application in breeding, but there are few reports on the mining and application of molecular markers related to circadian rhythm behavior traits. Compared with other animals, the mining and application of molecular markers is still a new field.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Screening method of molecular marker of circadian rhythm behavior related gene Cry1 of egg ducks

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The molecular marker and application of the gene Cry1 associated with the circadian rhythm behavior of laying ducks comprises the following steps:

[0020] 1. DNA extraction

[0021] Carry out DNA extraction with blood / cell / tissue genome extraction box to the laying duck blood sample (50 parts) that needs to detect, after DNA extraction, detect with 2% agarose gel electrophoresis, and utilize ultraviolet spectrophotometer to measure its OD value and sample Concentration, stored in -20 ℃ refrigerator. The DNA pool was constructed to finally obtain the mixed DNA pool of laying ducks.

[0022] 2. Primer Design

[0023] According to the duck Cry1 gene sequence (serial number is NW_004676465.1) published in the GeneBank database, use the software Oligo6.0 and Primer5.0 to design and screen primers, and send them to Biotechnology Co., Ltd. for synthesis. The primer sequence information is shown in Table 1:

[0024] Cry1 Gene PCR Amplification Primer Sequence

[0025]

...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a screening method of a molecular marker of a circadian rhythm behavior related gene Cry1 of egg ducks. The screening method comprises the following steps: (1) extraction of DNA: extracting DNA by using a blood / cell / tissue genome DNA kit, and then assaying the OD value and concentration; (2) designing of a primer: designing the primer by using Primer Primer 5.0, and delivering the primer to a biology company for synthesis; (3) PCR amplification: conducting PCR amplification by using a 20mu L reaction system and setting the PCR amplification conditions, and taking and detecting the product by agarose gel electrophoresis; (4) fragment purification of the amplification product, vector linking, conversion, positive clone screening and sequencing; and (5) SNP mutant site screening and correlation analysis for determining the circadian rhythm behavior related SNP of egg ducks. In the molecular breeding process, the mutant gene type of the circadian rhythm behavior related gene Cry1 detected by the method is regarded as the molecular marker.

Description

technical field [0001] The invention belongs to a poultry molecular marker selection method, in particular to a molecular marker selection method and application of the circadian rhythm behavior-associated gene Cry1 of laying ducks. Background technique [0002] Behavior, as a complex quantitative trait, is regulated by multiple genes and affected by various environmental factors. Among these various behaviors, the circadian rhythm behavior of poultry is often ignored by people, but it affects the welfare of poultry in large-scale intensive farming in Europe, America and China. It not only affects the egg production of poultry, but also affects the growth of poultry. Studies have shown that appropriate light intensity and time can make laying birds feel comfortable and can better develop the productivity of poultry, which is related to the cryptochrome gene Cry 1 to a certain extent. [0003] In the past, light was used as an environmental factor to study how it affects the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68C12N15/11C12N15/10
CPCC12Q1/6806C12Q1/6888C12Q2600/124C12Q2600/156
Inventor 杨胜林陆曼王单娜柳诚刚王旭平周美迪谭斌杨汝才
Owner GUIZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com