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Efficient and practical method for culturing freshwater chlorella and application thereof

A cultivation method and technology of chlorella, which are applied in the field of efficient and practical cultivation to promote the growth of freshwater chlorella, can solve the problem of low survival rate of algae under large-scale cultivation, limit the practical promotion and application of chlorella, and cannot play a role in regulating the water quality of aquaculture, etc. problem, to achieve the effect of small workload, small error and low cost

Inactive Publication Date: 2017-08-18
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the reasons that limit the application of chlorella in freshwater aquaculture can be summarized as follows: complex formula of culture medium, cumbersome operation of the cultivation process, high production cost, high requirements for water quality, low survival rate of algae under large-scale cultivation and other technical bottlenecks yet to break through
Most of the commercially available algae are cultured by heterotrophic fermentation. The algae cultivated by this culture method are used as water quality regulators and inoculated into aquaculture ponds. The photosynthetic autotrophy of chlorella leads to inability to survive, so it cannot play a role in regulating the water quality of aquaculture, which limits the actual application of chlorella in aquaculture ponds
[0005] For this reason, this patent develops an efficient and practical freshwater chlorella culture technology to overcome many defects such as the traditional chlorella formula is complicated, the operation is cumbersome, and it cannot reproduce and survive in the culture pond. The culture method has a simple formula, simplified operation, and can be applied Strong, high survival rate as algae species in aquaculture ponds, and strong adaptability to external conditions, low requirements for aquaculture water sources, rapid growth, short cultivation cycle, high yield per unit volume, easy to form dominant populations in aquaculture ponds, so that Effectively play a role in regulating water quality in aquaculture

Method used

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  • Efficient and practical method for culturing freshwater chlorella and application thereof
  • Efficient and practical method for culturing freshwater chlorella and application thereof
  • Efficient and practical method for culturing freshwater chlorella and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-5

[0042] A medium for large-scale cultivation of chlorella, mainly urea, potassium dihydrogen phosphate, magnesium sulfate, ferrous sulfate, EDTANa 2 , indole-3-acetic acid and ethephon mixture, and the concentration of each component is shown in Table 1.

[0043] Table 1 main composition list of main medium

[0044]

[0045] After mixing ingredients 1-5 of Examples 1-5 in the above Table 1, dissolve them in distilled water / aerated tap water according to the ratio of 1g:1000mL, and mix them uniformly to obtain solutions A, B, C, D, and E;

[0046] After mixing components 6 and 7 in Examples 1-5 in the above Table 1, dissolve them in 95% ethanol according to the ratio of 20mg: 1mL-10mL, and add A, B, C, D, E solutions in proportion by weight after mixing evenly , to obtain Chlorella culture solution A 1 , B 1 、C 1 、D 1,E 1 .

[0047] Dissolve Components 6 and 7 of Example 1 in the above table in distilled water at a ratio of 1mg:1mL, mix well and add to solution A in pr...

Embodiment 6-11

[0049] (1) Preparation: Chlorella culture solution A prepared in Example 1-5 1 , B 1 、C 1 、D 1 ,E 1 、A 2 According to the volume ratio of 1:1000, add it to the aerated tap water for 40 hours, and shake it well to obtain the culture water A containing the nutrients needed for the growth of chlorella 1-1 , B 1-1 、C 1-1 、D 1-1 ,E 1-1 、A 2-1 ;

[0050] (2) Inoculation: choose chlorella that grows vigorously, is pollution-free, and is in the exponential growth phase and inoculates them into the above-mentioned chlorella water A containing nutrients. 1-1 , B 1-1 、C 1-1 、D 1-1 ,E 1-1 、A 2-1 In, the initial density is 4.38×10 6 ind / mL (absorbance A 680 is 0.203) chlorella liquid F, G, H, I, J, K;

[0051] (3) Place the above-mentioned inoculated chlorella liquid F, G, H, I, J, and K in sealed plastic bags at 25°C and 70 μmol / m 2 ·S (light-to-dark ratio 12h:12h) under light conditions for cultivation.

[0052] The sealed plastic bag is placed on a shaker dedicated t...

Embodiment 12

[0058] Feeding test

[0059] Add the chlorella cultured in Example 6 to the crushed and sieved crucian carp feed raw material according to the mass ratio of 30%, and after mixing evenly, use a granulator to make pellet feed, dry it at 70°C and feed it to crucian carp. The daily feeding amount is 3%-5% of fish body weight.

[0060] The test crucian carp was stocked in a 300L aquarium respectively, and 20 fish were stocked in each aquarium. Three replicates were set up for each feed, and the pelleted feed not prepared with Chlorella culture solution B was set as the control group. After 8 weeks of breeding, the experimental fish were fasted for one day and then weighed. After dissection, muscle samples were taken for routine nutritional composition analysis, and liver samples were taken for non-specific immune and antioxidant indicators. The results are shown in Table 3-5.

[0061] Table 3 Effects of chlorella on growth performance and feed utilization of crucian carp

[0062...

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Abstract

The invention provides a culture medium for efficiently culturing chlorella. The culture medium is prepared from the following components in parts by weight: 50-80 parts of urea, 20-50 parts of monopotassium phosphate, 20-50 parts of magnesium sulfate, 0.5-1.5 parts of ferrous sulfate, 5-8 parts of EDTANa2 and 0.001-0.004 part of mixture of indole-3-acetic acid and ethephon. The invention further provides a method for culturing chlorella by utilizing the culture medium. After chlorella is continuously cultured for 5 days by the method, microscopic examination indicates that the chlorella has density of 35.26*10<6>ind / mL, and can be added into feed for feeding or adding into a culturing water body to improve the water quality. The chlorella culture solution contains six nutritional elements, is convenient to use, small in workload, convenient to store and deliver, and convenient for industrialized, standardized, marketing, commercialized and specialized production, and has accurate trace ingredient and element quantization and small error.

Description

technical field [0001] The invention belongs to the field of aquaculture, and in particular relates to an efficient and practical cultivation method for promoting the growth of freshwater chlorella and its application. [0002] technical background [0003] Chlorella is the earliest developed algae, as early as the early 1960s, Japan began factory production. After domestication, Chlorella can live in fresh water, sea water, and brackish water. There are three types of Chlorella ellipsoides, Chlorella chlorella and Chlorella pyrenoidosa, all of which can be artificially cultivated. [0004] In the past two decades, freshwater aquaculture has developed rapidly in the Bohai Rim region and even the whole country, and the development of chlorella as a seedling growth feed and water quality regulation for freshwater fish and prawns has become more and more important. Many farmers use chlorella to improve breeding methods and reduce the use of drugs to form a green ecological farm...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 张树林戴伟张达娟毕相东王泽斌
Owner TIANJIN AGRICULTURE COLLEGE
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