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Anti-bacterial film modified by silver-lysozyme nano-cluster

A nano-film, lysozyme technology, used in the preservation of human or animal bodies, chemicals for biological control, biocides, etc. and other problems, to achieve the effect of improving the antibacterial performance

Active Publication Date: 2017-08-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are mainly two methods for the preparation of antibacterial film materials based on silver nanoclusters: one is to form the nano silver antibacterial agent together with the resin through the plastic molding process, and this method has thermal stability to the antibacterial agent. The shortcomings of high antibacterial requirements and large dosage of antibacterial agents, especially for silver antibacterial agents, need to consider the cost issue; the other is to fix a layer of silver on the formed film by coating, spraying, etc. Antibacterial layer, the main problem that needs to be solved in this method is that the affinity between the antibacterial agent and the matrix material is low, which will cause problems such as low adsorption efficiency of the antibacterial agent and easy detachment from the surface of the carrier.

Method used

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  • Anti-bacterial film modified by silver-lysozyme nano-cluster

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Experimental program
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Effect test

Embodiment 1

[0035] The preparation method of silver-lysozyme nanoclusters is as follows: dissolve lysozyme in 10 mL of water at a concentration of 2-10 mg / mL, and then add 0.01-0.1 mmol AgNO 3 , Add NaOH solution under 500~1000rpm stirring condition to adjust the pH value of the solution to 8.0~12.0, then add 0.01mol / L NaBH respectively 4 Solution 50μL, 75μL, 100μL, 125μL and 150μL, react at room temperature under the same conditions for 1 hour. The wavelength of 450nm was selected as the excitation light to study the fluorescence characteristics of lysozyme-coated silver nanoclusters, the emission wavelength was from 500nm to 700nm, the excitation and emission slit was 5nm, and the voltage was high voltage. The results of fluorescence spectrometry of silver nanoclusters are as follows figure 1 As shown, after 1 hour of reaction, the fluorescence emission peak of the sample is located at 650 nm, indicating the formation of silver nanoclusters. With NaBH 4 With the increase of the addition a...

Embodiment 2

[0037] A method for preparing a highly efficient broad-spectrum silver / lysozyme nanocluster modified antibacterial film, the specific implementation steps are as follows:

[0038] (1) Clean the polyvinylidene chloride cling film material with ethanol and blow dry, soak it in a 0.05M Tris-HCl buffer solution containing 1mg / mL dopamine and pH=8.5, stir for 18 hours, and then remove it with deionization After rinsing with water for 2 minutes, it is dried to obtain a polydopamine modified nano film.

[0039] (2) Soak the polydopamine-modified base material in 10g / L glutaraldehyde solution and stir for 2 hours to combine one of the carbonyl groups of glutaraldehyde with the amino group of polydopamine, and rinse with deionized water for 2 minutes after removal After drying, the glutaraldehyde modified film material is obtained.

[0040] (3) Immerse the glutaraldehyde-modified nano film into the silver / lysozyme nanocluster solution prepared in Example 1, adsorb for 1 hour and take it out,...

Embodiment 3

[0043] A method for preparing a highly efficient broad-spectrum silver / lysozyme nanocluster modified antibacterial film, the specific implementation steps are as follows:

[0044] (1) Clean the polyvinylidene chloride cling film material with ethanol and blow dry, soak it in a 0.05M Tris-HCl buffer solution containing 5mg / mL dopamine and pH=8.5, stir for 18 hours, take it out and deionize Rinse with water for 5 minutes to obtain a polydopamine modified nano film.

[0045] (2) Soak the polydopamine-modified base material in 10g / L glutaraldehyde solution and stir for 2 hours to combine one of the carbonyl groups of glutaraldehyde with the amino group of polydopamine, and rinse with deionized water for 5 minutes after removal , To obtain glutaraldehyde modified film material.

[0046] (3) Immerse the glutaraldehyde-modified nano-film in the silver / lysozyme nanocluster solution, absorb for 1 hour, take it out, and rinse with deionized water for 5 minutes to obtain the silver / lysozyme na...

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Abstract

The invention discloses an anti-bacterial film modified by a silver-lysozyme nano-cluster, and belongs to the field of anti-bacterial materials. A preparation method of the anti-bacterial film comprises the following steps: modifying a layer of polydopamine on the surface of a substrate material; then, crosslinking a silver-lysozyme nano-cluster to the surface of the polydopamine through glutaraldehyde to obtain an efficient broad-spectrum antibacterial film material. According to different silver ion contents, the bacteriostasis rate of the anti-bacterial film on salmonella is 51.7 to 100 percent, and the bacteriostasis rate on staphylococcus aureus is 69.5 to 100 percent. In the anti-bacterial film, a substrate is modified by the silver-lysozyme nano-cluster, so that the stability of nano-silver is enhanced by adding lysozyme, the using amount of the silver is lowered greatly, the toxicity is lowered, the cost is controlled, and the reusing rate of the film is increased. The anti-bacterial film can be applied to preparation of food packaging materials and surface anti-bacterial films of medical anti-bacterial instruments.

Description

Technical field [0001] The invention relates to an antibacterial film modified by silver-lysozyme nanoclusters, belonging to the field of antibacterial materials. Background technique [0002] With the development of science and technology and the progress of society, people pay more and more attention to the health of their living environment, and infectious diseases caused by pathogenic microorganisms have always been one of the main threats to human health. Antibacterial film material refers to the assembly of different antibacterial agents inside or on the surface of the packaging material, so that the material itself has the performance of inhibiting the growth and reproduction of microorganisms. It not only has hygienic and self-cleaning functions, but also can effectively prevent bacterial cross-infection. Therefore, antibacterial films have a wide range of development and application prospects in food packaging, container inner surfaces, antibacterial films, biological ma...

Claims

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Application Information

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IPC IPC(8): A01N59/16A01N37/46A01N25/22A01P1/00C08J7/06C08L23/06C08L25/06C08L67/04
CPCA01N25/22A01N37/46A01N59/16C08J7/06C08J7/065C08J2323/06C08J2325/06C08J2367/04A01N2300/00
Inventor 方正梁丽陈思乌斯嘎勒李迪
Owner JIANGNAN UNIV
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