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SNP (single nucleotidepolymorphism) marker related to sow farrowing character as well as detection method and application thereof

A sow and marker technology, applied in the field of molecular biology, can solve the problem of inability to determine the main gene and its key mutation sites, and achieve the effect of important economic and social value

Inactive Publication Date: 2017-08-01
JIANGSU KANGLE FARMING
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Problems solved by technology

[0006] According to the website of the International Porcine QTL Database (http: / / www.animalgenome.org / cgi-bin / QTLdb / SS / index), QTLs affecting total litter size have been located on all autosomes and sex chromosomes of pigs , but most of them are QTLs located by microsatellite markers, and the confidence interval is mostly 10-20cM. It is impossible to determine the real main gene and its key variation sites, so it is difficult to directly apply it to the breeding and improvement of breeding pigs

Method used

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  • SNP (single nucleotidepolymorphism) marker related to sow farrowing character as well as detection method and application thereof
  • SNP (single nucleotidepolymorphism) marker related to sow farrowing character as well as detection method and application thereof
  • SNP (single nucleotidepolymorphism) marker related to sow farrowing character as well as detection method and application thereof

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Embodiment 1

[0030] 1. Extraction of Suhuai Sow Genomic DNA

[0031] Ear tissue samples from 347 Suhuai sows with accurate litter total litter size records were collected, placed in centrifuge tubes filled with 70% alcohol, and stored in a -20°C refrigerator for later use.

[0032] Using the traditional phenol / chloroform method to extract genomic DNA from ear tissue, the required reagents include:

[0033] Lysate laboratory equipment

[0034] Proteinase K (Germany MERCK Biotechnology Co., Ltd.)

[0035] Tris saturated phenol (Beijing Suolaibao Biotechnology Co., Ltd.)

[0036] Tris saturated phenol: chloroform: isoamyl alcohol (25:24:1) (Beijing Solaibao Biotechnology Co., Ltd.)

[0037] Chloroform (Jiangsu Yonghua Fine Chemicals Co., Ltd.)

[0038] Absolute ethanol (Guangdong Guanghua Technology Co., Ltd.)

[0039] 3M Sodium Acetate (Beijing Suolaibao Biotechnology Co., Ltd.)

[0040] The specific steps are as follows:

[0041] (1) Take a soybean-sized tissue sample, cut it into pi...

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Abstract

The invention discloses an SNP (single nucleotidepolymorphism) marker related to sow farrowing character as well as a detection method and application thereof. The locus of the SNP marker is a g. 95537674 nucleotide locus on a No.9 chromosome of an international pig genomic 10.2 version reference sequence, belongs to G / T mutation, and corresponds to the 255th site on the SEQ ID NO: 1; the SNP marker has significant correlation with the number of live births of sows. A primer pair for detecting the SNP marker provided by the invention comprises an upstream primer SEQ ID NO: 2 and a downstream primer SEQ ID NO: 3. The SNP marker provided by the invention is related to the farrowing performance of Suhuai sows, so that a high-yield sow strain can be screened through identifying the SNP marker, and the obtained high-yield sow strain has important economic benefit and social value.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to a SNP marker related to sow farrowing traits, a detection method and application thereof. Background technique [0002] The litter size of pigs is an important economic trait, and the increase of the litter size will greatly increase the supply of commercial pork and bring huge economic benefits to modern pig production. With the continuous and rapid development of my country's economy, people's living standards are gradually improving, and the demand for pork is also increasing. At the end of December 2014, the number of fertile sows in my country was about 43 million. If the sows gave birth to one more litter per litter on average, based on the calculation of two litters per sow per year, it would provide about 90 million more piglets for the entire industry each year. pig. Therefore, people are also paying more and more attention to how to improve the litter size perf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 李平华黄瑞华张倩李卫东
Owner JIANGSU KANGLE FARMING
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