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A kind of preparation method of k-carrageenan oligosaccharide with antioxidant activity

A technology of carrageenan oligosaccharides and antioxidant activity, applied in biochemical equipment and methods, methods based on microorganisms, preparation of test samples, etc., can solve problems such as no antioxidant activity, and achieve high product yield, The effect of stable quality and simple and fast preparation process

Active Publication Date: 2021-02-09
QINGDAO BZ OLIGO BIOTECH
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  • Description
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Problems solved by technology

There are reports on enzymatic hydrolysis of carrageenan oligosaccharides, but there is no report on enzymatic K-carrageenan oligosaccharides with antioxidant activity

Method used

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  • A kind of preparation method of k-carrageenan oligosaccharide with antioxidant activity
  • A kind of preparation method of k-carrageenan oligosaccharide with antioxidant activity
  • A kind of preparation method of k-carrageenan oligosaccharide with antioxidant activity

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Embodiment Construction

[0029] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments.

[0030] A preparation method of K-carrageenan oligosaccharides with antioxidant activity specifically comprises:

[0031] One, the preparation of K-carrageenase

[0032] 1. Inoculate the screened Cellulophagalytica strain N5-2 in the activation medium, and culture it on a shaker at 20-30°C and 150-250rpm / min for 15-20h, and then activate it to obtain a seed solution.

[0033] 2. Inoculate the seed liquid into the fermentation medium according to the inoculum amount of 2-4%, and culture on a shaker at 20-30°C and 150-250rpm / min for 15-20h.

[0034] 3. Refrigerate and centrifuge the fermentation broth at 4000-5000r / min for 20min at 4°C, and take the supernatant after centrifugation, which is the crude enzyme solution.

[0035] 2. Preparation of K-carrageenan oligosaccha...

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Abstract

The invention relates to the field of K-carrageenan oligosaccharides, in particular to a method for preparing K-carrageenan oligosaccharides with antioxidant activity. It includes the preparation of K-carrageenan enzyme and the preparation of K-carrageenan oligosaccharide, and also includes the determination of the ability to scavenge hydroxyl radicals of K-carrageenan oligosaccharide and the determination of the ability to scavenge superoxide anion of K-carrageenan oligosaccharide method. The preparation method of the invention is simpler and quicker than the existing preparation methods. The invention adopts enzymatic degradation, so that the preparation process is simple and quick, the yield of the product is high, and the quality is stable, and the product has anti-oxidation activity, and has the prospect of developing health food with anti-oxidation function.

Description

technical field [0001] The invention relates to the field of K-carrageenan oligosaccharides, in particular to a method for preparing K-carrageenan oligosaccharides with antioxidant activity. Background technique [0002] As a natural marine sulfated polysaccharide with unique structure, carrageenan is called heparan-like polysaccharide, and its biological activity has become a hot spot in the field of polysaccharide research. In recent years, the biological activity of carrageenan in physiological and pathological research has been discovered one after another. Experimental studies have shown that carrageenan has biological activities such as anti-angiogenesis, anti-tumor, immune regulation, anti-oxidation, antibacterial, anti-virus, and hypoglycemic. However, due to the large molecular weight of carrageenan polysaccharide, poor solubility and absorption, complex structure and other reasons, its application is limited. The molecular weight of carrageenan oligosaccharides i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/38C12P19/14G01N21/31G01N1/38G01N30/02G01N30/06C12R1/01
CPCC12N9/2468C12P19/14C12Y302/01083G01N1/38G01N21/314G01N30/02G01N30/06
Inventor 王丽丽张斌管昶徐小龙高芹芹岳帆
Owner QINGDAO BZ OLIGO BIOTECH
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