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Novel compound microbial agent and preparation method thereof

A compound microbial bacterial agent and a new technology, applied in the field of new compound microbial bacterial agent and its preparation, can solve the problems of complex production process, uneven effect, high production cost, achieve high-efficiency deodorization and fly killing effect, and reduce odor The effect of preventing product deterioration

Inactive Publication Date: 2017-07-21
HUNAN PUTAIER ENVIRONMENTAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex production process of the existing compound microbial bacterial agents, the effects are uneven, and the production cost is high, it is imminent to develop new effective, cheap and widely applicable compound microbial bacterial agents

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A new type of composite microbial bacterial agent, which is composed of active seed liquid and production medium in a volume ratio of 2:100; Yeast, Saccharomyces cerevisiae, Acetobacter pasteurianum, Lactobacillus plantarum, and Lactobacillus casei are composed of 5:2:1:1:1:1:1 by volume; the production medium is composed of the following ingredients: Every 10 tons of water contains 250kg of tomatoes, 250kg of bean sprouts, 140kg of wheat bran, 350kg of brown sugar, 300kg of molasses, 3kg of peptone, 3.5kg of yeast extract, and 3-9kg of glacial acetic acid.

[0031] The preparation method is:

[0032] Step 1, preparation of activated medium:

[0033] Add 10 g of peptone, 3 g of beef extract, 5 g of sodium chloride, and 20 g of agar into 1 L of distilled water, and mix well; adjust the pH to 7.0 with hydrochloric acid and sodium hydroxide; ;

[0034] Add 10g of peptone, 4g of yeast powder, 5g of beef extract, 20g of glucose, 5g of sodium acetate trihydrate, 2g of tria...

Embodiment 2

[0046] A new type of composite microbial bacterial agent, which is composed of active seed liquid and production medium in a volume ratio of 2:100; Yeast, Saccharomyces cerevisiae, Acetobacter pasteurianum, Lactobacillus plantarum, and Lactobacillus casei are composed of 10:3:2:1:1:1:1 by volume; the production medium is composed of the following ingredients: Every 10 tons of water contains 200kg of tomatoes, 200kg of bean sprouts, 100kg of wheat bran, 300kg of brown sugar, 250kg of molasses, 2kg of peptone, 2kg of yeast extract, and 3-9kg of glacial acetic acid.

[0047] The preparation method is:

[0048] Step 1, preparation of activated medium:

[0049] Add 9g of peptone, 2g of beef extract, 4g of sodium chloride, and 20g of agar per 1L of distilled water, and mix well; use hydrochloric acid and sodium hydroxide to adjust the pH to 7.0; ;

[0050] For each 1L of distilled water, add 9g of peptone, 3g of yeast powder, 4g of beef extract, 19g of glucose, 4g of sodium aceta...

Embodiment 3

[0062] A new type of composite microbial bacterial agent, which is composed of active seed liquid and production medium in a volume ratio of 2:100; Yeast, Saccharomyces cerevisiae, Acetobacter pasteurianus, Lactobacillus plantarum, and Lactobacillus casei are composed of 20:9:4:5:5:3:3 by volume; the production medium is composed of the following ingredients: Every 10 tons of water contains 300kg tomatoes, 300kg bean sprouts, 200kg wheat bran, 400kg brown sugar, 350kg molasses, 6kg peptone, 7kg yeast extract, and 3-9kg glacial acetic acid.

[0063] The preparation method is:

[0064] Step 1, preparation of activated medium:

[0065] Add 11g of peptone, 4g of beef extract, 6g of sodium chloride, and 20g of agar per 1L of distilled water, mix well; use hydrochloric acid and sodium hydroxide to adjust the pH to 7.0; sterilize at 122°C for 22min, and prepare beef extract peptone medium ;

[0066] Add 11g of peptone, 5g of yeast powder, 6g of beef extract, 21g of glucose, 6g of ...

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Abstract

The invention discloses a novel compound microbial agent. The novel compound microbial agent is prepared from an active seed fluid and a producing culture medium by volume ratio of 2 to 10. The active seed fluid is prepared from bacillus velezensis, camellia flower lactobacillus, Pichia kudriavzevii, brewer's yeast, acetobacter pasteurianus, lactobacillus plantarum and lactobacillus casei by volume ratio of (10-20) to (3-9) to (2-4) to (1-5) to (1-5) to (1-3) to (1-3). The producing culture medium is prepared from 200-300 kg of tomatoes, 200-300 kg of bean sprouts, 100-200 kg of wheat bran, 300-400 kg of brown sugar, 250-350 kg of molasses, 2-6 kg of peptone, 2-7 kg of yeast extract and 3-9 kg of glacial acetic acid which are contained in every 10 tons of water. The production and fermentation process is simple, the deodorization efficiency is high, microbial flora are stable, and he applicability is high.

Description

technical field [0001] The invention relates to a novel composite microbial bacterial agent and a preparation method thereof, which can be used for odor treatment in public toilets, garbage transfer stations, garbage landfills, domestic garbage storage points, sewage, slaughterhouses, breeding farms, and biological composting. Background technique [0002] With the growth of population and the continuous development of economy, people are increasingly aware of the importance of the environment to the sustainable development of human beings. At present, the annual production of domestic waste in my country is about 140 million tons, more than 80% of which are transported to landfills for landfill treatment. Organic domestic waste is decomposed by microorganisms to produce a large amount of NH 3 、H 2 S and other malodorous gases have caused great pollution to the landfill and the surrounding environment, affecting the health of sanitation workers and surrounding residents. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12R1/07C12R1/225C12R1/84C12R1/865C12R1/02C12R1/25C12R1/245
CPCC12N1/20C12N1/16
Inventor 胡峻吴小国马威李海龙陈林李安华
Owner HUNAN PUTAIER ENVIRONMENTAL CO LTD
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