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Device for tumor accurate treatment method based on CTC circulating tumor cells

A technology for tumor cells and treatment methods, which can be used in tissue cell/virus culture devices, measurement devices, biochemical cleaning devices, etc., and can solve problems such as easy generation of drug resistance

Pending Publication Date: 2017-07-18
郭昊伦
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] Aiming at the problem that drug resistance is easily produced in the current tumor treatment process, the present invention provides a device that can be used in a precise tumor treatment method based on CTC circulating tumor cells

Method used

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  • Device for tumor accurate treatment method based on CTC circulating tumor cells
  • Device for tumor accurate treatment method based on CTC circulating tumor cells
  • Device for tumor accurate treatment method based on CTC circulating tumor cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0111] Using the device described in the present invention, the present invention also provides a precise tumor treatment method based on CTC circulating tumor cells, comprising the following steps:

[0112] Step 1: Take 5-10ml of peripheral blood from a breast cancer patient

[0113] 5-10ml of peripheral blood is extracted from the patient, usually 7.5ml, and less than 5ml is not recommended. The storage and transportation of blood must be carried out within the recommended temperature range, that is, refrigerated at 4°C to 10°C, or at room temperature at 15°C to 30°C. Blood cannot be frozen, and it should be added to the first container.

[0114] Step 2: Isolation of CTC circulating tumor cells

[0115] The separation of CTC circulating tumor cells should be completed within a specified time after blood drawing, which generally varies according to different CTC separation methods, and may be several hours or several days. Some blood samples may coagulate and clot if the sp...

Embodiment 2

[0136] The second embodiment of the precise tumor treatment method based on CTC circulating tumor cells according to the present invention includes the following steps:

[0137] Step 1: Take 5-10ml of peripheral blood from prostate cancer patients

[0138] 5-10ml of peripheral blood is extracted from the patient, usually 7.5ml, and less than 5ml is not recommended. The storage and transportation of blood must be carried out within the recommended temperature range, that is, refrigeration at 4°C to 10°C, or room temperature at 15°C to 30°C. Blood cannot be frozen.

[0139] Step 2: Isolation of CTC circulating tumor cells

[0140] The separation of CTC circulating tumor cells should be completed within a specified time after blood drawing, which generally varies according to different CTC separation methods, and may be several hours or several days. Some blood samples may coagulate and clot if the specified time is exceeded, even in the presence of an anticoagulant.

[0141] ...

Embodiment 3

[0159] The third embodiment of the precise tumor treatment method based on CTC circulating tumor cells according to the present invention includes the following steps:

[0160] Step 1: Take 5-10ml of peripheral blood from patients with colon cancer

[0161] 5-10ml of peripheral blood is extracted from the patient, usually 7.5ml, and less than 5ml is not recommended. The storage and transportation of blood must be carried out within the recommended temperature range, that is, refrigeration at 4°C to 10°C, or room temperature at 15°C to 30°C. Blood cannot be frozen.

[0162] Step 2: Isolation of CTC circulating tumor cells

[0163] The separation of CTC circulating tumor cells should be completed within a specified time after blood drawing, which generally varies according to different CTC separation methods, and may be several hours or several days. Some blood samples may coagulate and clot if the specified time is exceeded, even in the presence of an anticoagulant.

[0164]...

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Abstract

The invention provides a device for a tumor accurate treatment method based on CTC circulating tumor cells. The device comprises CTC circulating tumor cell separation equipment for separating the CTC circulating tumor cells from added peripheral blood, CTC circulating tumor cell detection equipment for detecting the separated CTC circulating tumor cells, closable CTC circulating tumor cell culture equipment for carrying out in-vitro culture and proliferation on the separated CTC circulating tumor cells, and drug sensitivity detection equipment for carrying out a drug sensitivity experiment on the cultured CTC circulating tumor cells. According to the device, an in-vitro drug sensitivity test is carried out on the CTC circulating tumor cells of a patient and the device has an individualized accurate treatment effect on the patient; a parallel drug sensitivity test method is carried out and one preferred clinical medication scheme can selected each time so that the tumor cells are attacked by different types of medicines all the time, and furthermore, the medicine adaptation capability of tumors become weak and the problem of drug resistance caused by single clinical medication can be avoided.

Description

technical field [0001] The invention belongs to the medical field, and relates to a device that can be used in a precise tumor treatment method based on CTC circulating tumor cells. Background technique [0002] Tumor is a major disease in the world today. Its high mortality rate and the complexity of treatment have prompted countries all over the world to spend a lot of manpower and material resources on research, but so far no breakthrough has been made. It was recognized by American medical scientist Siddhartha Mukherjee Siddhartha Mukherjee is known as the king of all diseases. [0003] Tumor formation is a complex process with many factors, which makes its research more complicated. The treatment of tumors is based on the understanding of tumors, and has undergone surgery, radiotherapy, conventional chemotherapy and targeted drug therapy, as well as the current popular research method immune-immune cell therapy. [0004] Surgery, as the name suggests, is the removal o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/00C12M1/34C12Q1/02
CPCG01N33/5011C12M45/00
Inventor 郭昊伦
Owner 郭昊伦
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