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Method and device for preparing hydrogel microspheres

A technology of hydrogel microspheres and water-in-oil, which is applied to chemical instruments and methods, laboratory containers, laboratory utensils, etc., can solve the problems of large particle size distribution range and poor repeatability, and achieve a reliable particle size. control effect

Inactive Publication Date: 2017-07-14
SHANGHAI TURTLE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the most widely used and most mature technology is the preparation of micron-sized hydrogel microspheres by reverse-phase suspension polymerization, but the particle size distribution range of microspheres produced by reverse-phase suspension polymerization is large and the repeatability is poor. Therefore, how to prepare monodisperse micron The application of high-grade hydrogel microspheres in the field of highly accurate bioanalysis is a key problem to be solved urgently

Method used

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  • Method and device for preparing hydrogel microspheres
  • Method and device for preparing hydrogel microspheres
  • Method and device for preparing hydrogel microspheres

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] In this example, acrylamide monomer is used as a water-soluble monomer, methylenebisacrylamide is used as a crosslinking agent, VA-086 is used as an initiator, and water is used as a pore-forming agent to prepare hydrogel microspheres with a swelling degree of 6 times.

[0020] Concrete preparation steps are as follows:

[0021] (1) Preparation of aqueous mixture: Weigh acrylamide monomer (0.6g), methylenebisacrylamide (0.036g) and VA-086 (0.012g) into a 5mL centrifuge tube, and measure 2.5mL 2mM The above mixture was dissolved in EDTA aqueous solution, mixed by vortex mixer, and ultrasonically dissolved, and the volume was adjusted to 4 mL to obtain an aqueous mixture.

[0022] The aqueous mixture was transferred to a vial with a silicone stopper inserted into a nitrogen-displaced needle. Nitrogen gas was slowly introduced to remove oxygen for 20 minutes. After the nitrogen replacement was completed, 300 μL of mineral oil was added.

[0023] (2) Preparation of oily ...

Embodiment 2

[0028] In this example, carboxyacrylamide monomers were used as water-soluble monomers to prepare hydrogel microspheres. The composition of the raw materials is shown in Table 1 below, and the preparation steps are the same as those in Example 1, so they will not be described again.

[0029] Table 1 Embodiment 2 raw materials and consumption table

[0030] Component Dosage Water-soluble monomer: acrylic acid 0.84mL Water-soluble monomer: acrylamide 0.36g Neutralizer: 20% sodium hydroxide solution 1.75mL Cross-linking agent: methylene bisacrylamide 0.012g Initiator: potassium persulfate 0.02g mineral oil Same as Example 1 Surfactant: Abil EM90 Same as Example 1 Pore ​​former: water Dilute to 4mL

Embodiment 3

[0032] In this example, hydrogel microspheres were prepared by using methylol acrylamide monomer as a water-soluble monomer. The composition of the raw materials is shown in Table 1 below. The collected micro-droplets were cured at 70° C. for 12 hours. The other preparation steps were the same as those in Example 1, so they will not be repeated here.

[0033] Table 2 Embodiment 3 raw materials and consumption table

[0034]

[0035]

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Abstract

The invention belongs to the technical field of hydrogel polymer microsphere preparation and relates to a method for preparing hydrogel microspheres based on a microfluidic chip technology and a device for implementing the preparation method. According to the method, an aqueous mixture of a water-soluble monomer, a crosslinking agent, an initiator and a pore-forming agent is used as a dispersion phase, an oil mixture of a water-insoluble liquid and lipophilic hydrophilic amphiphilic molecules is used as a continuous phase, the aqueous mixture and the oil mixture form micro-scale water-in-oil droplets in a microfluidic chip, the droplets are collected, cured and demulsified and monodisperse micro-scale hydrogel microspheres are recovered. The monodisperse micro-scale hydrogel microspheres have the advantages of controllable and uniform grain diameters and high recoverability and can be widely used in technical fields of gene immobilization and screening, protein and cell coating, and a drug slow release system.

Description

technical field [0001] The invention relates to the technical field of droplet generation and preparation of hydrogel polymer microspheres, in particular to a method and device for preparing hydrogel microspheres based on microfluidic chip technology. Background technique [0002] Hydrogel is a cross-linked macromolecular polymer with a three-dimensional network structure, which can swell in water and retain a large amount of water without dissolving, and molecules can diffuse in the hydrogel. The research of polymer hydrogel microspheres in the fields of medical engineering and biochemical engineering has been particularly popular in recent years. Due to its hydrophilicity, porosity, high specific surface area and excellent biocompatibility, hydrogel microspheres can be used as microstructural units, microseparators, microreactors, and micromemory in DNA, cells, and proteins. The embedding of bioactive drugs, the coating of bioactive drugs, the immobilization of enzymes, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/56C08F22/38C08F220/06C08F220/58C08F222/14C08F2/32C08F2/01B01L3/00
CPCB01L3/502769C08F2/01C08F2/32C08F220/06C08F220/56C08F222/385C08F220/58C08F222/102
Inventor 张京红闫哲刘艳景奉香
Owner SHANGHAI TURTLE TECH
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