A kind of Chinese herbal medicine preparation and method for shrimp immunity enhancement and sterilization with stable medicinal properties
An immune enhancer and shrimp technology, applied in the field of aquatic compound feed additives, can solve the problems of drug residues, economic losses in shrimp breeding industry, serious diseases, etc., and achieve the effects of high feeding, enhancing immunity and promoting feeding.
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Embodiment 1
[0026] The preparation method of the compound Chinese herbal medicine immune enhancer and the basal feed comprises the following steps:
[0027] First, prepare the active ingredients of Chinese herbal medicine. After uniformly pulverizing the flaky Astragalus membranaceus, pass through an 80-mesh sieve, mix with distilled water according to the mass volume ratio of 1:10, decoct twice, each time for 2 hours, combine the filtrate twice, and concentrate under reduced pressure , add an appropriate amount of 95% ethanol so that the final alcohol content is 80%, overnight, suction filtration, to obtain astragalus polysaccharide;
[0028] Evenly pulverize the flake Eucommia ulmoides, pass through a 80-mesh sieve, mix with ethyl acetate (containing 0.05mol / L hydrochloric acid) according to the mass volume ratio of 1:8, stir and reflux on a water bath at 65°C for 3 hours, and repeat the extraction 2 times, the combined filtrates were separated to separate ethyl acetate to obtain chloro...
Embodiment 2
[0034] Effects of Compound Chinese Herbal Medicine Immunity Enhancer on Non-specific Immunity of Litopenaeus vannamei
[0035] In this experiment, the inventor fed the two kinds of Chinese herbal immune enhancers prepared in Example 1 to Litopenaeus vannamei, and measured its non-specific immune indicators, aiming to screen out the non-specific immune function and pathogen resistance of Litopenaeus vannamei. The ability of the compound Chinese herbal medicine immune enhancer.
[0036] 1 Materials and methods
[0037] 1.1 Materials
[0038]The Litopenaeus vannamei used in the test was purchased from the Shazikou Shrimp Farm in Laoshan District, Qingdao City, with an average body weight of 7.45 ± 0.41 grams. The basic feed for the test consisted of 34% fish meal and 20% soybean meal in terms of mass fraction. , 16.4% of peanut meal, 22% of wheat flour, 5% of soybean lecithin, 0.6% of multivitamins, and 2% of compound minerals. The nutrient components in the feed were calculat...
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