Perfusion system for in-vitro extracellular electroneurophysiological research

Abstract

The invention provides a perfusion system for in-vitro extracellular electroneurophysiological research, belonging to the technical field of apparatus for in-vitro extracellular electroneurophysiological research. The perfusion system provided by the invention overcomes the technical problem of too great errors during quantitative analysis of transmembrane movement of ions confined in and located out of a cell wall in virtue of a perfusion tank. According to a technical scheme in the invention, a perfusate is stored in a perfusate storage device and is communicated with the perfusion system via a perfusate inlet pipe; a peristaltic pump is arranged between the perfusate storage device and the perfusion system; after the perfusate passes through the perfusion system, the perfusate becomes a waste perfusate which is communicated with a waste perfusate collecting device via a waste perfusate discharge pipe; the waste perfusate discharge pipe is provided with a waste-perfusate-discharge control pump; the perfusion system comprises a mounting rack and a perfusion device; and the perfusion device is mounted on the mounting rack. The perfusion system provided by the invention enables the perfusate to enter from the bottom of a perfusion tank, and the flow rate of the perfusate is reduced after obstruction by a screen mesh, so an ion flow rate during efflux of an in-vitro sample is reduced due to influence by flowing of the perfusate; and thus, the quantitative analysis of the transmembrane movement of ions confined in and located out of the cell wall is improved in precision.

Description

technical field [0001] The invention belongs to the technical field of devices for electrophysiological research on extracellular nerves in vitro, in particular to a perfusion system for electrophysiological research on extracellular nerves in vitro. Background technique [0002] MED64 Planar Microelectrode Array Recording System is a multi-channel in vitro extracellular neurophysiological research tool produced by Japan Alpha Med Science Company. The system includes a complete set of hardware equipment consumables and analysis software required for in vitro electrophysiological research. Users can conveniently and quickly perform electrophysiological detection on isolated materials such as brain slices and cultured nerve cells; they can measure local electrical changes directly adjacent to the outside of cells, and record the electrophysiological signals of multiple cells in parallel. The MED64 device can record and stimulate different sites at the same time; and the MED64 ...

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Problems solved by technology

[0004] In order to overcome the deficiencies in the prior art and solve the technical problem of excessive error caused by using the perfusion tank to quantitatively analyze the ion transmembrane movement of the inner and outer walls of the cell in the prior art, the present invention provides simple structure, convenient use and can reduce A perfusion system for the electrophysiological study of isolated extracellular nerves in which the perfusate interferes with the environment of the extracellular wall of the sample

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