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Preparation method of pineapple leaf extract applied to field of skin whitening

A technology of pineapple leaves and extracts is applied in the field of inhibiting tyrosinase, ether extracts of pineapple leaf alcohol extracts and their preparation, achieving strong inhibitory effect and solving the effects of resource waste

Active Publication Date: 2017-07-07
HAINAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about its inhibition of tyrosinase activity, and it is expected to be used as a whitening agent in the field of whitening cosmetics.

Method used

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  • Preparation method of pineapple leaf extract applied to field of skin whitening
  • Preparation method of pineapple leaf extract applied to field of skin whitening
  • Preparation method of pineapple leaf extract applied to field of skin whitening

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Tyrosinase Promotes the Determination of the Best Activity Conditions for L-Tyrosine to Generate Melanin

[0035] (1) Effect of temperature on the optimal activity of tyrosinase

[0036] Take 8 clean and dry brown volumetric flasks of 10mL and divide them into 4 groups, 2 in each group, and mark them as C1 and C2 respectively. Add each test solution according to Table 1:

[0037] Table 1 System composition and the addition amount of each reagent

[0038]

[0039]

[0040] Add the corresponding amount of buffer solution and L-tyrosine solution to the 4 sets of volumetric flasks according to the above table as the substrate of the reaction system, then add the corresponding amount of potato extract, and place the 4 sets of solutions at 0°C, 10°C, At 30°C and 40°C, after reacting for 35 minutes, measure the absorbance immediately (the measurement wavelength is 475nm is the best). Such as figure 1 .

[0041] (2) The influence of system reaction time on ...

Embodiment 2

[0067] (1) Take 1 kg of dried pineapple leaves, crush them, add 5 L of methanol-ethanol-isopropanol mixed solvent (the volume ratio of the three is 3:6:1), soak and extract 4 times at 40-50°C, each time The extraction time is 6 hours;

[0068] (2) merging the extracts obtained by soaking and extracting each time in step (1) to obtain the extract, and filtering to remove the solid residue to obtain the filtrate;

[0069] (3) After the filtrate obtained in step (2) was concentrated under reduced pressure to remove the organic solvent, 2.5L of water was added to disperse it, and then extracted twice with petroleum ether, using 5L of petroleum ether each time;

[0070] (4) The petroleum ether phases after the extraction of step (3) are combined, concentrated under reduced pressure, and spray-dried to obtain 3.9g of pineapple leaf extract (GC-MS such as Figure 6 ).

Embodiment 3

[0072] (1) Take 3kg of dried pineapple leaves, crush them, add 60L of methanol-ethanol-isopropanol mixed solvent (the volume ratio of the three is 3:6:1), soak and extract twice at 40-50°C, each time The extraction time is 8 hours;

[0073] (2) merging the extracts obtained by soaking and extracting each time in step (1) to obtain the extract, and filtering to remove the solid residue to obtain the filtrate;

[0074] (3) After the filtrate obtained in step (2) was concentrated under reduced pressure to remove the organic solvent, 20L of water was added to disperse it, and then extracted 4 times with petroleum ether, using 20L of petroleum ether each time;

[0075] (4) The petroleum ether phases extracted in step (3) were combined, concentrated under reduced pressure, and spray-dried to obtain 13.7 g of pineapple leaf extract.

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Abstract

The invention relates to a preparation method of a pineapple leaf extract applied to field of skin whitening and provides a preparation method of a pineapple leaf extract. The method is characterized by comprising the following steps of (1) taking dry pineapple leaves, crushing the dry pineapple leaves, adding the dry pineapple leaves into a solvent, soaking and extracting at 40-50 DEG C for 2-4 times, wherein each extraction time is 6-8h and the mass-volume ratio (kg / L) of the pineapple leaves to the solvent is 1:(5-20); (2) merging an extract liquid obtained by soaking and extracting each time in the step (1) to obtain an extract solution, filtering and removing a solid residue to obtain filtrate; (3) carrying out vacuum concentration on the filtrate obtained in the step (2) to remove an organic solvent, adding water for dispersion, and extracting the filtrate by using petroleum ether for 2-4 times; and (4) merging a petroleum ether phase extracted in the step (3), carrying out vacuum concentration and drying to obtain the pineapple leaf extract.

Description

technical field [0001] The invention belongs to the field of natural extraction, and in particular relates to an ether extract of pineapple leaf alcohol extract, a preparation method thereof and an application for inhibiting tyrosinase. Background technique [0002] Melanin (melanin) is mainly produced by melanocytes in the human body, which can reduce the damage of ultraviolet rays to the skin; however, the abnormal accumulation of melanin will cause hyperpigmentation, and easily cause freckles, age spots, and melasma. The researchers found that tyrosinase plays an important role in the biosynthesis of melanin. In the process of melanin biosynthesis, tyrosinase plays a very important catalytic role. Tyrosinase can promote the conversion of L-tyrosine into L-dopa, and L-dopa is converted into dopaquinone through oxidation, and dopaquinone multipolymerizes to form melanin. At present, there are mainly two ways to inhibit the production of melanin: one is to prevent the prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9794A61Q19/02
CPCA61K8/97A61K2800/782A61K2800/805A61Q19/02
Inventor 纪明慧郭飞燕陈光英吕纪行
Owner HAINAN NORMAL UNIV
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