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Device and method for producing propionic acid and coproduced vitamin B12 by means of semi-continuous fermentation

A vitamin and B12 technology, applied in the field of bioengineering, can solve the problems of difficult production, difficulty in propionibacterium, low expansion bed filling rate, etc.

Active Publication Date: 2017-07-04
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the problems existing in the prior art such as difficulty in industrial scale-up production, low expansion bed filling rate, difficulty in separating propionic acid bacteria from propionic acid fermentation broth, etc., the purpose of the present invention is to provide a semi-continuous fermentation for the production of propionic acid and co-production of vitamin B 12 The device and method, the method is based on membrane separation of cells, coupled with fixed-bed chromatography technology, adsorption and separation of propionic acid in the fermentation broth, the feedback inhibitory effect of propionic acid on fermentation is relieved, and the method of re-inoculation is adopted, On the basis of fed-batch fermentation, the semi-continuous fermentation co-production of vitamin B12 and propionic acid is realized, which improves the fermentation efficiency and substrate conversion rate and reduces the fermentation cost

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  • Device and method for producing propionic acid and coproduced vitamin B12 by means of semi-continuous fermentation
  • Device and method for producing propionic acid and coproduced vitamin B12 by means of semi-continuous fermentation
  • Device and method for producing propionic acid and coproduced vitamin B12 by means of semi-continuous fermentation

Examples

Experimental program
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Effect test

Embodiment 1

[0083] A semi-continuous fermentative production of propionic acid and co-production of vitamin B 12 devices such as figure 1 As shown, the device includes a fermentation unit, a membrane separation unit, a propionic acid separation unit and vitamin B 12 The conversion unit, the outlet of the fermentation unit passes through the membrane separation unit and the propionic acid separation unit and vitamin B 12 The feed port of the conversion unit is connected, and the discharge port of the propionic acid separation unit is connected with the reflux port of the fermentation unit;

[0084] Described fermenting unit comprises fermentor 11, and the upper half of the tank body of described fermentor 11 is provided with fermentor feed inlet 12, and the tank top of described fermentor 11 is provided with stirring device 13, and described feed inlet 12 Used for the supplement of feed liquid and the addition of nutrients;

[0085] The propionic acid separation unit includes a chromato...

Embodiment 2

[0092] Example 2: Production of propionic acid and co-production of vitamin B by semi-continuous fermentation 12 Methods

[0093] (1) Fermentation culture of Propionibacterium freunderi

[0094] Activation culture of strains: activate the strains stored in glycerol tubes or slopes by streaking on a plate, and place them in constant temperature anaerobic culture at 30°C for 72 hours;

[0095]Seed liquid culture: Take the activated slant seeds and insert them into the liquid medium, and culture them at 30°C for 48 hours;

[0096] Fermentation tank culture: Inoculate 10% of the inoculum into a 10L fermenter, culture at 30°C, 50r / min, add high-concentration glucose when the glucose concentration is lower than 10g / L, and adjust the pH to 6.8 with ammonia water -7.2, regularly take samples to measure the OD600 value of the bacteria. When the fermentation lasted for 84 hours, the intracellular vitamin B12 metabolic intermediate Ado-cbi accumulated to the maximum value, at this tim...

Embodiment 3

[0107] Example 3: Production of propionic acid and co-production of vitamin B by semi-continuous fermentation 12 Methods

[0108] (1) Fermentation culture of Propionibacterium freunderi

[0109] Activation culture of strains: activate the strains stored in glycerol tubes or slopes by streaking on a plate, and place them in a constant temperature anaerobic culture at 35°C for 40 hours;

[0110] Seed liquid culture: Take the activated slant seeds and insert them into the liquid medium, and culture them at 35°C for 42 hours;

[0111] Fermentation tank culture: Inoculate 10% of the inoculum into a 10L fermenter, culture at 35°C and 30r / min, add high-concentration glucose when the glucose concentration is lower than 10g / L, and adjust the pH to 6 with ammonia water -8, take samples regularly to determine the OD600 value of the bacteria. When the fermentation proceeded to 80 hours, the intracellular vitamin B12 metabolic intermediate Ado-cbi accumulated to the maximum value, at th...

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Abstract

The invention provides a device and a method for producing propionic acid and coproduced vitamin B12 by means of semi-continuous fermentation. The device comprises a fermentation unit, a membrane separation unit, a propionic acid separation unit and a vitamin B12 conversion unit, wherein a discharge hole of the fermentation unit is connected with a feeding hole of the propionic acid separation unit and a feeding hole of the vitamin B12 conversion unit by the membrane separation unit; a discharge hole of the propionic acid separation unit is connected with a reflux inlet of the fermentation unit; the membrane separation unit comprises a membrane separation device; the membrane separation device comprises a membrane core; the aperture of the membrane core is 0.1-0.22mu m. The method and the device not only effectively overcomes the feedback inhibition action of the propionic acid for fermentation, but also overcomes the disadvantages that an expanded bed is low in filling efficiency and difficult to enlarge, solves the problem that the addition of DMB has adverse effects on resource utilization of fermentation wastewater, and realizes the cyclic utilization of the fermentation wastewater.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a semi-continuous fermentation for producing propionic acid and co-producing vitamin B 12 Device and method, especially related to membrane separation technology and chromatography technology coupling fermentation co-production of vitamin B 12 and propionic acid process. Background technique [0002] Propionibacterium freudenreichii (Propionibacterium freudenreichii) is a feed microbial strain approved by the US FDA and the American Association of Feed Control Officials (AAFCO), and is also a production strain for anaerobic fermentation of vitamin B12. The anaerobic fermentation process of vitamin B12 has low power consumption and has been adopted by most manufacturers. However, in recent years, with the gradual increase in the level of aerobic fermentation of vitamin B12 by Pscudomonas denitrificans, the advantages of the anaerobic fermentation process have been unable to...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12P7/52C12P19/42C12R1/01
CPCC12M23/58C12M43/00C12P7/52C12P19/42
Inventor 王自强徐国霞魏立全王云山苏志国
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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