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A cordyceps militaris strain liquid fermentation method

A technology for liquid fermentation and Cordyceps militaris, which is applied to microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems of cumbersome preparation method of fermentation medium, increase the workload of extracting cordycepin, and consume manpower and material resources, etc. The effect of increasing development value, increasing mycelial growth metabolites, and increasing content

Inactive Publication Date: 2017-06-30
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
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AI Technical Summary

Problems solved by technology

Due to the advantages of liquid fermentation over solid fermentation in terms of fermentation scale, cell growth rate, growth density and controllability, the extraction of cordycepin by liquid fermentation of Cordyceps militaris is the main application method for preparing cordycepin nowadays, and the fermentation method is relatively mature. However, the content of cordycepin extracted by the current fermentation method is maintained at a stable level, and it is difficult to make new breakthroughs under the limitations of the existing technological conditions. The preparation method of the fermentation medium used is relatively cumbersome and difficult to operate. It also increases the workload of extracting cordycepin, consumes manpower and material resources, and is not economical and environmentally friendly.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The method of liquid state fermentation of Cordyceps militaris strain is as follows:

[0033] 1) Collect wild Cordyceps militaris sporocarps in Zuojiashan District, Jilin City in early and mid-September each year, and select robust sporocarps for separation and purification. The medium is PDA medium, and liquid fermentation is carried out after successful separation and purification;

[0034] 2) Preparation of liquid fermentation medium: Freshly picked mature ginseng fruit is squeezed by a juicer, and the ginseng seeds and peel are removed by coarse filtration. Bacteria for 20 minutes to obtain the ginseng fruit juice fermentation substrate;

[0035] 3) Inoculate the strains obtained in step 1) into the ginseng fruit juice fermentation substrate prepared in step 2), and cultivate in a shaker with the temperature controlled at 19° C. and the shaker speed at 200 rad / min. After 8 days of dark culture , Determination of the content of cordycepin and saponin in the fermenta...

Embodiment 2

[0039] The method of liquid state fermentation of Cordyceps militaris strain is as follows:

[0040] 1) Collect wild Cordyceps militaris sporocarps in Zuojiashan Mountain, Jilin City in early and mid-September each year, and select robust sporocarps for separation and purification. The medium is PDA medium, and liquid fermentation is carried out after successful separation and purification;

[0041] 2) Preparation of liquid fermentation medium: Freshly picked mature American ginseng fruit is squeezed by a juicer, and the ginseng seeds and pericarp are removed by coarse filtration. The filtrate is homogenized to obtain ginseng fruit juice. Put 100mL ginseng fruit juice in a 250mL triangular bottle, and heat it at 125°C. Sterilize for 10 minutes to obtain the American ginseng fruit juice fermentation substrate;

[0042] 3) Inoculate the strain obtained in step 1) into the ginseng fruit juice fermentation substrate prepared in step 2), and cultivate in a shaker with the temperatu...

Embodiment 3

[0045] The method of liquid state fermentation of Cordyceps militaris strain is as follows:

[0046] 1) Collect wild Cordyceps militaris sporocarps in Zuojiashan Mountain, Jilin City in early and mid-September each year, and select robust sporocarps for separation and purification. The medium is PDA medium, and liquid fermentation is carried out after successful separation and purification;

[0047] 2) Preparation of liquid fermentation medium: ginseng mustaches were processed into small pieces of about 2 cm, then pulverized with a traditional Chinese medicine grinder, and the powder was passed through an 80-mesh sieve, 1 g of ginseng powder was added to 30 mL of water, refluxed for 3 hours, and then centrifuged at 8000 rad / min for 15 minutes to obtain Add 8g of glucose to 1L of the supernatant water extract, put 100mL of the water extract into a 250mL conical flask, sterilize at 120°C for 20min, and obtain the fermentation substrate of the water extract of ginseng straight bea...

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Abstract

A cordyceps militaris strain liquid fermentation method is provided. The method includes steps of inoculating a liquid fermentation medium with a cordyceps militaris strain, performing shaking culture with the temperature being 19-21 DEG C and the rotary speed of a shaking table being 200-220 rad / min, and performing dark culture for 8-14 d. The liquid fermentation medium is prepared from fruit or root of araliaceae plants. According to the method, the fruit or root of araliaceae plants is mainly adopted as a raw material of the medium, the raw material is easily available, addition of other components is not needed, a preparing process is simple, convenient, economical and environment-friendly, and a fermentation product by adopting the specially prepared medium is increased in cordycepin content and contains a large amount of rare saponin components. A novel operating route for cordyceps militaris strain liquid fermentation is provided. The method deserves energetic popularization and application and fills gaps of related techniques.

Description

technical field [0001] The invention relates to the field of fermentation of Cordyceps militaris strains, in particular to a method for liquid fermentation of Cordyceps militaris strains. Background technique [0002] Cordyceps militaris is Ascomycotina, Ergotaceae, Ergotaceae, and Cordyceps genus. It is a complex composed of two parts: the subterranean base and the fruiting body part above the ground. In the worm body, the larva absorbs nutrients, and the larvae are filled with hyphae and die. In the summer and autumn of the second year, grass bodies grow from the larvae, and wild Cordyceps militaris can be collected around mid-September. Cordycepin contained in Cordyceps militaris has a variety of physiological activities, such as anti-tumor, anti-virus, and immune-enhancing effects. [0003] In the prior art, chemical synthesis and biosynthesis are generally used to obtain cordycepin. The production cost of chemical synthesis is relatively high, the synthesis process i...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12P19/40C12P33/20C12R1/645
CPCC12P1/02C12P19/40C12P33/20C12N1/145C12R2001/645
Inventor 闫梅霞张宝香王英平张瑞郭靖许世泉
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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