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Method for combining power-frequency electromagnetic field generating device with platinum-based drugs

A generation device and platinum-based drug technology, applied in the field of biotechnology and medical treatment, can solve the problems of lack of unified and complete understanding, complex biophysical mechanism of action, etc., and achieve uniform magnetic field strength, convenient implementation, and reduced dose effects.

Inactive Publication Date: 2017-06-30
ZHEJIANG UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the biophysical mechanism of the magnetic field is complex, not a single molecular pathway, so there is no unified and complete understanding of the biological effects of the magnetic field, including the effect of inhibiting tumor cells

Method used

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  • Method for combining power-frequency electromagnetic field generating device with platinum-based drugs
  • Method for combining power-frequency electromagnetic field generating device with platinum-based drugs
  • Method for combining power-frequency electromagnetic field generating device with platinum-based drugs

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Example 1, the combination method of the magnetic field generating device of the present invention and platinum-based drugs

[0023] This method is realized through the following steps: build a power frequency electromagnetic field generating device, use a power frequency 50 / 60Hz power supply, and continuously change the voltage through a DC and AC autotransformer, thereby generating superimposed static (from DC) and alternating currents inside the irradiation part. (from AC) electromagnetic field, the average strength of the loaded magnetic field is 5.5 millitesla (mT), and the total irradiation time for the irradiated object on the irradiated part is 60-120 minutes per day; For the irradiated object, platinum drugs such as cisplatin, carboplatin, oxaliplatin and other platinum drugs are selected. Administered 3 times a week, this is the lower dose selected within the effective dose range of cisplatin.

[0024] see figure 1, the power frequency electromagnetic field ...

Embodiment 2

[0030] Example 2, the present invention acts on the cultured Wilms tumor cells G401, the control group, the irradiation group, the cisplatin group, and the combination group are treated separately, and each treatment condition has 3 duplicate wells, and the cells treated the day before are collected every day , count with a living cell counter, calculate the number of cells according to the readings, and make a cell proliferation curve and an electromagnetic field inhibition curve for cells.

[0031] The specific implementation process is as follows:

[0032] Cell line and culture method: Wilms tumor cell G401, which is an adherent cell, was cultured in McCoy medium containing 10% fetal bovine serum in an incubator at 37°C, containing 5% carbon dioxide and saturated water vapor.

[0033] Main equipment: power frequency electromagnetic field generator, cell incubator, living cell counter.

[0034] Cell viability test: Wilms tumor cell G401 was seeded on a 6-well plate at a den...

Embodiment 3

[0037] Example 3, the present invention acts on the cultured neuroblastoma cell CHLA255, the control group, the irradiation group, the cisplatin group, and the combination group are treated separately, and each treatment condition has 3 duplicate wells, and the cells treated the day before are collected every day , count with a living cell counter, calculate the number of cells according to the readings, and make a cell proliferation curve and an electromagnetic field inhibition curve for cells.

[0038] The specific implementation process is as follows:

[0039] Cell line and culture method: Neuroblastoma cell CHLA255, which is an adherent cell, was cultured in McCoy medium containing 10% fetal bovine serum in an incubator at 37°C, containing 5% carbon dioxide and saturated water vapor.

[0040] Main equipment: power frequency electromagnetic field generator, cell incubator, living cell counter.

[0041] Cell viability experiment: Neuroblastoma cells CHLA255 were seeded in 6...

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Abstract

The invention provides a method for combining a power-frequency electromagnetic field generating device with platinum-based drugs. A power-frequency electromagnetic field generating device is constructed and a power supply with a power frequency is 50 / 60Hz is employed; voltages are changed continuously by direct-current and an alternating-current autotransformers, so that within a superimposed static and alternating electromagnetic field is generated inside an irradiation component and the average loaded magnetic field intensity is 5.5 milli tesla; the total irradiation time of an irradiated object on the irradiation component each day is 60 to 120 mins; and platinum-based drugs are applied on the irradiated object on the irradiation component. The device is designed reasonably; with a unique power frequency and simultaneous loading of static and alternating electromagnetic fields, the intensity of the magnetic field generated by the device is uniform, the height is controllable, and implementation becomes convenient. The interference on the irradiated object is low and no trauma is caused. Experiments in vivo and vitro demonstrates that curative effects of independent usage of the platinum-based chemotherapeutic drug and the magnetic field can be enhanced and several kinds of tumours can be suppressed obviously based on combined usage of the oncotherapy electromagnetic field and the platinum-based chemotherapeutic drug; the dosage of cis-platinum can be reduced; and the toxic and side effects can be reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology and medical technology, relates to the interdisciplinary fields of physics, biology and medicine, and in particular relates to a combination method of a power frequency electromagnetic field generating device and platinum drugs. Background technique [0002] The frequency of low-frequency electromagnetic field is within 300 kilohertz (kHz). It has no thermal effect but has biological effect. The mechanism is still unknown, and it may be related to acting on charged macromolecules and changing their functional state. The tumor suppressive effect of magnetic fields has been widely reported (see Artacho-Cordón Francisco et al., 2013, Int J Mol Sci; Tofani S, 2015, Curr Topics in Med Chem). The European Union and the US FDA have approved tumor therapeutic magnetic field generating equipment (Novocure Company of Israel) is used for the clinical treatment of glioblastoma multiforme, and the technical feat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61N2/04A61M37/00G01N33/50C12Q1/02A61K33/24A61K31/282A61P35/00
CPCA61N2/02A61K31/282A61K33/24A61M37/00A61M2037/0007G01N33/5008
Inventor 陈希袁林清王璨王金湖朱坤李华美
Owner ZHEJIANG UNIV
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