Method for increasing contents of lycopene and lutein in carrots
A technology of lycopene and carrots, applied in the direction of preservation of fruits/vegetables by freezing/refrigeration, etc., can solve the problems of multiple freeze-thaw, fluctuations in ambient temperature, slow biochemical reactions, etc., and achieve the effect of good practicability
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[0016] Carrots are washed, dried and pre-processed. Thin slices with a thickness of 10 ± 1 mm and a diameter of 32 ± 2 mm were cut from the middle of the carrot. First, put -70 o Quick-frozen in the refrigerator of C, then put into -20 o C in the freezer for 72 h, and finally, at 4 o C refrigerator for 4 h, and this freeze-thaw cycle was repeated five times. Use fresh samples as a control for analysis and detection.
[0017] Determination of carotenoids: Add 6.3 mL of methanol and 700 μL of 60% potassium hydroxide solution to 100 mg of freeze-dried carrot powder, stir and mix, and put the mixture in 60 o C in a hot water bath for 30 min. Subsequently, 6 mL of Tris buffer (containing 50 Mm Tris solution, 1 M NaCl solution, pH 7.5) was added to the mixture, stirred, at 4 o C for 10 min, then added 16 mL of chloroform, stirred, and stood on ice for 10 min, then the mixture was centrifuged at 4,000 rpm for 15 min at 4 °C. This process is repeated twice to ensure maximum ext...
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