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A method for cultivating cold-resistant maize varieties using maize allopolyploid

An allopolyploid, corn technology, applied in the directions of botanical equipment and methods, applications, plant genetic improvement, etc., can solve problems such as corn production loss, and achieve the effects of improving yield, strong cold resistance, and expanding germplasm resources

Active Publication Date: 2019-03-01
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problem that the low-temperature chilling injury of corn causes losses to corn production, the purpose of the present invention is to provide the use of corn allopolyploids in cultivating cold-resistant corn varieties

Method used

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  • A method for cultivating cold-resistant maize varieties using maize allopolyploid
  • A method for cultivating cold-resistant maize varieties using maize allopolyploid
  • A method for cultivating cold-resistant maize varieties using maize allopolyploid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Cultivation of cold-resistant maize inbred lines using maize allopolyploid

[0029] Follow the steps below:

[0030] (1) In the spring of 2011, MTF-1 (Tripsazea creammaize T.2n=76) was propagated by division, cuttings or other vegetative methods at the Wenjiang Breeding Base of Sichuan Agricultural University Chengdu Campus when the ground temperature rose above 10°C Seedlings were obtained, and planted as a single plant according to the row spacing of 2m×2m; at the same time, corn inbred line Zheng 58 (Zea mays L., 2n=20) was sown in 10 batches, with 20 plants in each batch, and an interval of 3 days between each batch; Publicly available MTF-1 biomaterials from Sichuan Agricultural University

[0031] (2), bag the MTF-1 ear before silking, cut the filament to 2-3cm after silking, pollinate the MTF-1 ear with the pollen of Zheng 58, every 2 days after the first pollination Pollination again, a total of 3 pollinations, mark on the paper bag after each pollina...

Embodiment 2

[0036] Example 2 Cultivation of Cold-resistant Maize Inbred Lines Using Maize Allopolyploid

[0037] Using MTF-1 as the non-recurrent parent and B73 and Chang 7-2 as the recurrent parent, according to the breeding method described in Example 1, the improved B73 line with strong cold resistance and the improved Chang 7-2 with strong cold resistance were bred respectively. Tie.

Embodiment 3

[0038] Embodiment 3: Contrast test of plasma membrane permeability measurement (conductivity method) after low temperature treatment of the improved system

[0039] (1) Test materials

[0040] (1), the Zheng 58 improved line bred in Example 1.

[0041] (2), Zheng 58 (control).

[0042] (3), the improved B73 line bred in Example 2.

[0043] (4), B73 (control).

[0044] (5), the Chang 7-2 improved line bred in Example 2.

[0045] (6), Chang 7-2 (control).

[0046] (2) Test method

[0047] Pick 100 plump seeds and place in a Petri dish Germinate the seeds. Disinfect the seeds with 0.5% sodium hypochlorite solution for 5 minutes, then rinse with distilled water for 3 times; divide into 3 equal parts, soak overnight at room temperature (about 25°C) so that the seeds can absorb water, and place the seeds evenly on a petri dish with wet filter paper after 12 hours middle. Add distilled water to each petri dish, the depth of the water layer is 1mm; put it in a 25°C incubator...

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Abstract

The invention belongs to the field of maize breeding methods, provides application of a maize allopolyploid in cultivation of a cold-resistant maize variety, and further provides a method for cultivating the cold-resistant maize variety by using the maize allopolyploid. According to the method, the maize allopolyploid is used as a cold-resistant donor and a distant hybridization bridge material, and the maize variety with high cold resistance is obtained through backcross transformation and cold resistance authentication. The method solves the problem of difficulty in distant hybridization of maize and closely related genera thereof, provides a new way for using the cold resistance of the closely related genera of the maize in maize production and expands cold-resistant germplasm resources of the maize; meanwhile, the variety selected through the method disclosed by the invention is high in cold resistance; a maize planting area in a cold district can be enlarged, and the yield of the maize in the cold district can be increased; moreover, the method for cultivating the cold-resistant maize variety has the advantages of low cost and high efficiency.

Description

technical field [0001] The invention belongs to the field of corn breeding methods, and in particular relates to a method for cultivating cold-resistant corn varieties by using corn allopolyploid. Background technique [0002] Corn (Zea mays L.) is a temperature-loving crop. When the temperature is lower than 10°C, the plant growth is obviously slow, and the growth stops at 6°C-8°C. When the temperature is lower, the cells and tissues are irreversibly damaged. Corn germination and seedling stages are very sensitive to low temperature (Ma Yanhua et al. Crop Journal, 2012, (4) 1-8). Symptoms of low temperature damage during germination and seedling stage are usually browning and wilting of mesocotyls and coleoptiles, water-soaked and hypoplastic leaves, and even failure to survive due to stunted growth of seedlings (Tan Zhenbo et al. Maize Science, 2002, 10 (2): 56-60). Chilling injury is the main meteorological disaster in corn producing areas in northern my country. The No...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 唐祈林何如钰李晓锋严旭李杨李燕赵艳丽王红林谢孟林穆罕默德·扎法尔·伊克巴尔李静孙汝龙马行云温小冬
Owner SICHUAN AGRI UNIV
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