Composition and kit for detecting the content of donkey hide source component in tortoise shell gelatin, deer horn gelatin and fake gelatin, and detection method thereof
A technology of deer antler glue and tortoise shell glue, which is applied in the direction of measuring devices, material separation, and material analysis, can solve the problem that the components derived from donkey skin do not exclude the components derived from horse hide, cannot be quantitatively detected, and cannot be relatively stable, etc. question
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Embodiment 1
[0034] Example 1 Detection of pure glue
[0035] 1. Materials and reagents
[0036] Material: all kinds of pure glue, including donkey skin, horse skin, and cowhide skins, including donkey skin, horse skin, and cowhide skins, including donkey skin glue, horse skin glue, yellow gelatin, pig skin glue, sheep skin glue, tortoise shell glue, antler glue, and fish skin glue , Pigskin, sheepskin, tortoise shell, antlers and fish skins are decocted, concentrated and dried.
[0037] Polypeptide I (shown in SEQ ID NO. 1), polypeptide II (shown in SEQ ID NO. 2), and polypeptide III (shown in SEQ ID NO. 3) were synthesized by a biological company.
[0038] Reagents: ammonium bicarbonate (analytical purity), trypsin (sequence purity).
[0039] 2. Detection method
[0040] 1) Preparation of matrix solution: Weigh 0.50g of the reference substance test matrix into a 250ml measuring flask, and add a small amount of 1% NH 4 HCO 3 Solution (pH8.0), ultrasonic to completely dissolve the sample, use 1% NH ...
Embodiment 2
[0057] Example 2 Detection of mixed glue samples
[0058] 1. Materials and reagents
[0059] Material: The mixed gum sample is made from the tortoise shell gum and the pure product gum sample of deer horn gum in Example 1 by adding different quality donkey-hide glue, including tortoise shell glue containing 15% (w / w) donkey-hide glue, 30% (w / w) ) The tortoise shell glue of Ejiao and the deerhorn glue containing 30% (w / w) of Ejiao were used as samples 1-3, respectively.
[0060] Polypeptide I (shown in SEQ ID NO. 1), polypeptide II (shown in SEQ ID NO. 2), and polypeptide III (shown in SEQ ID NO. 3) were synthesized by a biological company.
[0061] Fish skin glue (reference substance detection matrix) was prepared according to the method described in Example 1.
[0062] Reagents: ammonium bicarbonate (analytical purity), trypsin (sequence purity).
[0063] 2. Detection method
[0064] 1) Preparation of matrix solution: Weigh 0.50g of the reference substance test matrix into a 250ml measu...
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