Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Foot and mouth disease virus inhibitor using lithium chloride as effective component

A technology for foot-and-mouth disease virus and active ingredients, which is applied in the directions of alkaline/alkaline earth metal chloride active ingredients, antiviral agents, etc., to achieve the effects of clear and single ingredients, low cost and simple preparation

Active Publication Date: 2017-06-13
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether LiCl has an impact on FMDV and h

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Foot and mouth disease virus inhibitor using lithium chloride as effective component
  • Foot and mouth disease virus inhibitor using lithium chloride as effective component
  • Foot and mouth disease virus inhibitor using lithium chloride as effective component

Examples

Experimental program
Comparison scheme
Effect test

Example

[0026] Test example 1 Cytotoxicity detection of LiCl

[0027] In order to detect the effect of LiCl on normal cell growth, the cytotoxicity of LiCl was tested using the Cell Counting Kit-8 (CCK8) kit. The specific operation steps are as follows: Transfer BHK-21 cells that grew well on DMEM cell culture medium to a 96-well cell plate. After the cells covered a single layer, discard the cell culture medium, wash 3 times with PBS solution, and then add 100 μL Drugs with serial concentrations (dilute LiCl to 10, 20, 30, 40, 60, 80 and 100 mmol / L with serum-free cell culture medium), set up three replicates of drugs with different concentrations, and set up a negative control (without Add drug) and blank control (no cells, only add culture medium), put them in a 37°C incubator for 48h. After rinsing with PBS, add 100 μL of 10% CCK8 solution to each well, continue to incubate in a 37°C incubator for 1-4 hours, and measure the absorbance value of each well at 450 nm with a microplat...

Example

[0029] Test example 2 Determination of the antiviral activity of LiCl against foot-and-mouth disease virus

[0030] The antiviral activity of LiCl against FMD virus was determined by the following protocol and verified by the following experiments. The specific operation steps are as follows: Transfer the BHK-21 cells that grew well on DMEM cell culture medium to 24-well cell plate. After the cells covered a single layer, discard the cell culture medium, wash 3 times with PBS solution, and inoculate each well. FMDV (MOI=0.1) was added with series concentrations of LiCl (10, 20, 30, 40 mmol / L, respectively), and three replicates were set up. At the same time, a control group without drug addition but inoculated with FMDV was set up. Incubate in a 37°C incubator for 48 hours. Discard the virus liquid, wash three times with pre-cooled PBS to remove free virus, add 400 μL of culture medium to each well, put it in a -20°C refrigerator for 3 times, and collect the virus liquid, cen...

Example

[0032] Test Example 3 Detection of FMDV Inhibition of Viral Replication Stage

[0033] 1. Virus adsorption stage

[0034]In order to test whether LiCl has an effect on virus adsorption to BHK-21 cells, the BHK-21 cells were transferred to a 24-well plate. After the cells covered a monolayer, the culture medium was discarded, and 200 μL of FMDV virus solution was added to each well (MOI= 0.1), then add 200 μL of different concentrations of LiCl to each well, so that the final concentrations of LiCl are 10, 20, 30 and 40 mmol / L respectively, set up a negative control without adding drugs, and put the cell plate in a refrigerator at 4 °C for 1 h , allowing only the virus to attach to the cell surface. Discard the virus solution, wash away the free virus with PBS, add 400 μL of culture solution to each well, put it in a -20°C refrigerator for 3 times, freeze and thaw repeatedly, collect the virus solution, centrifuge at 10,000 rpm for 10 minutes to remove cell debris, and use the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a foot and mouth disease virus inhibitor using lithium chloride as the effective component and belongs to the field of medicine. The foot and mouth disease virus inhibitor is characterized in that the action concentration of the lithium chloride is not higher than 40mmol/L, the inhibition effect of the lithium chloride on foot and mouth disease viruses is the most evident in the concentration range of 20-40mmol/L, the inhibition effect occurs in the replication stage of foot and mouth disease virus infection, and the inhibition effect occurs in the early state of foot and mouth disease virus replication. The foot and mouth disease virus inhibitor has the advantages that the lithium chloride is clear and single in component and evident in inhibiting effect on foot and mouth disease virus infection, and a foundation is laid for and convenient conditions are provided for application of the lithium chloride to foot and mouth disease virus molecular or cytological studies and medicine antiviral effect researches.

Description

technical field [0001] The invention relates to the use of chemical substances in the field of medicine, in particular to a foot-and-mouth disease virus inhibitor with lithium chloride as an active ingredient. Background technique [0002] Foot-and-mouth disease (FMD) is an acute, febrile, highly contagious disease caused by foot-and-mouth disease virus (FMDV), which can infect domestic animals and wild artiodactyls, including cattle. , pigs, sheep, camels and deer etc. The wide host range and rapid spread of foot-and-mouth disease have attracted the attention of the International Organization for Animal Health since reports of foot-and-mouth disease being susceptible to accidental or intentional transboundary spread in all countries of the world. In view of the fact that FMD can cause huge economic losses and social impact, the World Organization for Animal Health (OIE) and the Food and Agriculture Organization of the United Nations (FAO) list it as the first category of a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K33/14A61P31/14
CPCA61K33/14
Inventor 赵付荣常惠芸
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com