Common-path interference phase microscopy one-time imaging system and method

Abstract

The invention provides a common-path interference phase microscopy one-time imaging system and method. The common-path interference phase microscopy one-time imaging system comprises a laser, a neutral adjustable damper, a beam-expanding aligner, a half transparent and half reflecting mirror, a field diaphragm, an unpolarized beam splitter prism, a reflecting objective table, a sample, a microscope objective, a third lens, a CCD (Charge Coupled Device) and a computer. The common-path interference phase microscopy one-time imaging system is based on a differential interference light path, the unpolarized beam splitter prism is used for partitioning laser light into two beams of parallel light, namely, object light and reference light, the reflecting objective table is used for reflecting the object light and the reference light totally, the object light and the reference light are combined through the unpolarized beam splitter prism and transmitted in a common-path way, and the combined light is collected by a CCD camera and transmitted to the computer to be displayed after being amplified through the microscope objective. By adopting the common-path interference phase microscopy one-time imaging system and method, the conventional path interference imaging system can be realized by adjusting the angle of the reflecting objective table, including off-axis interference, co-axial interference and slight off-axis interference. The system has a high practical value and a wide application prospect on the aspect of phase microscopy, and can be particularly applied to the field of biological cell morphological identification.

Description

technical field [0001] The invention belongs to the research field of biological cell imaging technology, and in particular relates to a primary imaging system and method of a common optical path interference phase microscope. Background technique [0002] Biological samples under observation, such as living cells, can be treated as transparent, and these cells often appear as phase objects. In order to observe these phase objects clearly, it is common practice to convert phase information into intensity distribution, which leads to the emergence of phase microscopic imaging. In the last ten years, since various quantitative phase microscopy imaging techniques have been continuously proposed, it provides an extremely important research method for studying the tissue structure and dynamic behavior of phase objects similar to biological cells, and Phase microscopic imaging technology basically works by applying the interference principle of light, so this kind of technology c...

AI Technical Summary

Problems solved by technology

For example, in the patent CN 104034257 A, a Fizeau-type quasi-common optical path structure synchronous phase-shift interferometry device and method, this technology solves the problem of synchronous phase-shift interference used in the common optical path interference system, so that the existing optical elements in the system The phase measurement error caused by the stress birefringence effect is greatly reduced; another example is the patent CN 102221327 A phase-shift interference microscopy device and method based on Zernike phase contrast imaging. This invention solves the problem that the existing Zernike phase contrast imaging can only be used for Limitations of qualitative observation, while overcoming the technical problems of existing interference microscopy methods such as sensitivity to environmental vibrations, poor stability, high coherent noise, and low lateral resolution

However, these interference microscopy devices require a lot of optical components during use, especially the microscope system, the experimental cost is high, and the requirements for the professionalism of the users are high, so the application threshold is relatively high

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