Composite microbial agent and preparation method and application thereof

Abstract

The invention relates to a composite microbial agent and a preparation method and application thereof, and belongs to the technical field of organic waste treatment. The preparation method of the composite microbial agent comprises the steps of activating first degrading bacteria and second degrading bacteria, and preparing multiple beef extract-peptone solid mediums; inoculating activated degrading bacteria into different beef extract-peptone solid mediums for cultivation according to the type of the contained strain separately, and selecting the first degrading bacteria and the second degrading bacteria in a log phase or a stable phase for dilution separately to obtain first bacterial suspension and second bacterial suspension; and mixing the first bacterial suspension and the second bacterial suspension to obtain the composite microbial agent, wherein the first degrading bacteria comprise bacillus and streptococcus, and the second degrading bacteria comprise pseudomonas, acidophile and acinetobacter. The preparation method is simple and easy to operate and short in consumed time. The composite microbial agent obtained through the method is used for treating organic wastes, and has efficient and stable degradation capacity on oxytetracycline, tetracycline, aureomycin and doxycycline contained in the organic wastes.

Description

technical field [0001] The invention relates to the technical field of organic waste treatment, and in particular to a composite bacterial agent and its preparation method and application. Background technique [0002] Microbial fermentation is a commonly used low-input, high-efficiency harmless treatment method for organic waste. At present, most of the organic wastes are piled and fermented naturally, the composting time is long, the composting temperature is low, and the killing and deodorizing effects on harmful microorganisms and parasite eggs are not ideal, and long-term composting leads to an increase in anaerobic fermentation, denitrifying bacteria A large number of multiplication, a large amount of nitrogen loss. Therefore, adding effective microorganisms during the accumulation of organic waste can not only accelerate the decomposition of organic carbon, reduce the loss of nitrogen, reduce the volatilization of ammonia and hydrogen sulfide during the accumulation ...

AI Technical Summary

Problems solved by technology

[0003] At this stage, there are some microbial agents used to treat organic waste, but the sources of bacteria contained in some of these microbial agents are limited, the price is expensive, and it is not suitable for general use; the bacteria contained in some agents Complicated, difficult to control the preparation process and conditions

Moreover, most of the existing microbial agents only have the ability to degrade one or two of oxytetracycline, tetracycline, aureomycin and doxycycline contained in organic waste, and there is no one that can simultaneously degrade them. A microbial agent with good degradation effects on oxytetracycline, tetracycline, aureomycin and doxycycline