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Acquisition method of initial buds of ailanthus

A method for obtaining Ailanthus altissii tree, which is applied to the field of obtaining initial buds of Ailanthus sinensis tree, can solve the problems of difficulty in explant disinfection, low incidence of initial buds, poor activity of sprouts, and the like, and achieves rapid development and the incidence of initial buds. High, sprout elongation effect

Inactive Publication Date: 2017-05-31
唐春艳 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims at the technical problems such as difficulty in disinfection of explants, browning, low incidence of initial buds, and poor activity of sprouts in the existing tissue culture of Ailanthus ailanthus, and provides a method for preparing aseptic explants of Ailanthus ailanthus and inducing initial buds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] In a sunny climate for at least 3 consecutive days, the year-old shoots that grew vigorously on the periphery of the canopy of the Ailanthus ailatid tree were collected as explants. After the explants were rinsed with sterile water, they were soaked in a broad-spectrum fungicide with a volume concentration of 0.1% for 30 min, and the explants were taken out, soaked in 70% alcohol for 1 min, and then moved into Soak in sodium hypochlorite solution with a volume concentration of 5% for 20 minutes, take it out and put it in a mixture of 10% hydrogen peroxide and 2 drops of Tween, stir and soak for 30 minutes, and finally wash it with sterile water for 3 times, each time for 5 minutes ; cut the explants into at least 1 axillary bud, 1.5-2.0cm long stem segments, classify and put them in containers according to the degree of lignification of the stem segments, and set aside; inoculate the explants obtained after the disinfection treatment in the induction medium ; The induct...

Embodiment 2

[0022] In a sunny climate for at least 3 consecutive days, the year-old shoots that grew vigorously on the periphery of the canopy of the Ailanthus ailatid tree were collected as explants. After the explants were rinsed with sterile water, they were soaked in a broad-spectrum fungicide with a volume concentration of 0.2% for 25 min, and the explants were taken out and soaked in 70% alcohol for 2 min, and then moved into Soak in sodium hypochlorite solution with a volume concentration of 6% for 15 minutes, take it out and put it in a mixture of 15% hydrogen peroxide and 4 drops of Tween, stir and soak for 25 minutes, and finally wash it with sterile water for 3 times, each time for 5 minutes ; Cut the explants into stem segments with at least 1 axillary bud and a length of 2.0-2.5 cm, classify them into containers according to the degree of lignification of the stem segments, and put them in containers for later use; inoculate the explants obtained after disinfection into the in...

Embodiment 3

[0025] In a sunny climate for at least 3 consecutive days, the year-old shoots that grew vigorously on the periphery of the canopy of the Ailanthus ailatid tree were collected as explants. After the explants were rinsed with sterile water, they were soaked in a broad-spectrum fungicide with a volume concentration of 0.3% for 20 min, and the explants were taken out and soaked in 70% alcohol for 2 min, and then moved into Soak in sodium hypochlorite solution with a volume concentration of 8% for 10 minutes, take it out and put it in a mixture of 25% hydrogen peroxide and 5 drops of Tween, stir and soak for 20 minutes, and finally wash it with sterile water 4 times, each time for 3 minutes Cutting the explants into stem segments with at least one axillary bud and a length of 2.5-3 cm, and placing them in containers according to the degree of lignification of the stem segments, and placing them in containers for subsequent use; inoculating the explants obtained after disinfection i...

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Abstract

The invention discloses an acquisition method of initial buds of ailanthus. The method comprises the following steps: preparing explants, sterilizing the explants, and inducing initial buds, wherein newborn ailanthus twigs are collected as explants, the explants are sterilized to obtain sterile explants, and the sterile explants are inoculated into an initial bud induction culture medium to be cultured for 20 to 30 days in an appropriate environment to obtain ailanthus initial buds with rapid growth and vigorous activity. The newborn ailanthus twigs are selected as a tissue culture propagation material, and a sterilizing way of matching various chemicals with a given concentration in a given sterilizing time is adopted, so that the acquisition rate of the sterile explants is high and can reach 90 percent or more, the induction culture medium and the culture way are optimized, the browning of the explants is reduced, the germination rate of the initial buds is high, the buds are rapid in growth and vigorous in activity, and the economic benefit and social benefit are good.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for obtaining initial buds of Ailanthus ailanthus. Background technique [0002] Ailanthus altissima (Mill.) Swingle, Simaroubaceae (Simaroubaceae) Ailanthus genus plant, deciduous tree, up to more than 20 meters high, smooth bark with straight lines; tender branches are pith, yellow or yellow when young Yellow-brown pilose, shedding later. Light-loving, able to adapt to a wide range of climatic conditions, resistant to low temperatures of -30 ℃; resistant to drought and barrenness, strong adaptability, except clay, can grow in various soils and neutral, acidic and alkaline soils, suitable for deep, fertile, humid of sandy soil. Cold-resistant, drought-tolerant, not water-humidity resistant, long-term water accumulation will cause rotten roots and death, deep rooting. It has strong resistance to smoke and sulfur dioxide, less pests and disease...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 唐春艳陈素云
Owner 唐春艳
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