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A method for environmental safety assessment of transgenic Artemisia annua

A technology of environmental safety and evaluation method, applied in the field of safety evaluation of transgenic plants, can solve problems such as unreported research on transgenic artemisia annua, and achieve a good demonstration effect.

Active Publication Date: 2020-05-05
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] To date, many transgenic plants have been field tested and, some have been commercialized; however, experiments evaluating the environmental release of transgenic Artemisia annua have not been reported.

Method used

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  • A method for environmental safety assessment of transgenic Artemisia annua
  • A method for environmental safety assessment of transgenic Artemisia annua
  • A method for environmental safety assessment of transgenic Artemisia annua

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Experimental program
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Effect test

Embodiment

[0056] Embodiment A method for environmental safety assessment of transgenic Artemisia annua, comprising the following steps:

[0057] 1. Prepare the experimental site for the evaluation of agronomic traits

[0058] According to China’s GMO safety assessment regulations, Artemisia annua was planted in a rectangular experimental site covering an area of ​​about 0.3 hectares, 100 m long and 30 m wide; wild-type recipient maize was planted around each area to isolate GYR and Wild-type recipients; all plants were cultivated in corresponding areas according to conventional methods, and about 2304 plants were planted in each area (the row spacing was 1.25m).

[0059] 2. Detection of agronomic traits

[0060] The transgenic Artemisia annua GYR and wild-type recipients were grown in the greenhouse for 2 months, and then transplanted to the field to continue to grow. After transplantation, the number of dead seedlings was counted. Three months after transplantation, the growth and mo...

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Abstract

The invention provides an environmental safety evaluation method of transgenic shoots. The method is characterized in that the agronomic characters, the stress resistance and the gene flow of the transgenic shoots are tested; meanwhile, wild type receptor shoots are treated as the contrast; the parameters set in the evaluation cover various indexes of difference of the transgenic shoots and the wild type receptor shoots; on this basis, a model is built for the environmental release of the transgenic shoots, and the important demonstration significance on explaining the substantial equivalence is brought. According to the method, an evaluation system built based on the abovementioned three parts is simple to operate, easy to achieve, and high in intuition and result accuracy. The method provides testing indexes for the environmental safety evaluation of the transgenic shoots and also provides the technical support for the commercial risk evaluation of the transgenic shoot varieties.

Description

technical field [0001] The invention belongs to the field of safety evaluation of transgenic plants, and in particular relates to an environmental safety evaluation method of transgenic Artemisia annua. Background technique [0002] Malaria is a vector-borne disease caused by Plasmodium. Malaria is prevalent in 102 countries and regions. According to World Health Organization (WHO) estimates, there are 2 billion people living in disease areas, especially in some countries in Africa, Southeast Asia, and Central and South America, where the mortality rate of falciparum malaria is extremely high. The World Health Organization (WHO) in the African region has the highest burden of the disease, estimating that malaria accounts for 90% of all deaths and that children under 5 account for 78% of malaria deaths. [0003] Currently, the most effective treatments for malaria are artemisinin-based combination therapies. Artemisinin is a sesquiterpene lactone drug isolated from Artemisi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68A01G7/00A01G7/06
CPCA01G7/00A01G7/06C12Q1/6895C12Q2600/13
Inventor 刘华唐雪明王金斌白蓝蒋玮吴潇潘爱虎贾军伟
Owner SHANGHAI ACAD OF AGRI SCI
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