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Method of preparing sugar solution

A manufacturing method and technology of sugar solution, applied in biochemical equipment and methods, glycosylase, biofuel, etc., can solve the problems of increased cost and high enzyme cost, and achieve the effect of suppressing manufacturing cost and improving the reduction effect

Active Publication Date: 2017-05-24
TORAY IND INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the method of producing sugar liquid using glucoamylase is expensive, the cost for producing sugar liquid is high

Method used

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  • Method of preparing sugar solution

Examples

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Embodiment

[0092]Examples are given below to describe the present invention concretely. However, the present invention is not limited by these Examples.

reference example 1

[0093] (Reference Example 1) Preparation of cellulase (culture solution) derived from filamentous bacteria

[0094] Cellulase (culture solution) derived from filamentous fungi was prepared as follows.

[0095] [pre-culture]

[0096] Corn steeping liquid 25% (w / vol), glucose 2% (w / vol), ammonium tartrate 0.37% (w / vol), ammonium sulfate 0.14 (w / vol), calcium dihydrogen phosphate 0.2% (w / vol ), calcium chloride dihydrate 0.03% (w / vol), magnesium sulfate heptahydrate 0.03% (w / vol), zinc chloride 0.02% (w / vol), iron (Ⅲ) chloride hexahydrate 0.01 % (w / vol), copper sulfate (II) pentahydrate 0.004% (w / vol), manganese chloride tetrahydrate 0.0008% (w / vol), boric acid 0.0006% (w / vol), and heptamolybdic acid Add hexammonium tetrahydrate 0.0026% (w / vol) to distilled water, pour 100mL of distilled water containing the above components into a 500mL Erlenmeyer flask with baffles, and autoclave at 121°C for 15 minutes . After standing to cool, PE-M and Tween80 separately autoclaved at 121...

reference example 2

[0099] (Reference Example 2) Method for Measuring Activity of Cellulase Derived from Filamentous Fungi

[0100] The enzymatic activity of cellulase derived from filamentous fungi was measured and evaluated by the method shown below as the activity of the enzyme group involved in cellulolysis, as the activity of cellobiose and endoglucanase (1) 4 - Decomposition activity of nitrophenyl-β-D-lactopyranoside (pNP-Lac), measured and evaluated as β-glucosidase activity by the method shown below (2) 4-nitrophenyl-β- The decomposition activity of D-glucopyranoside (pNP-Glc), as the activity of endoxylanase and xylosidase involved in the decomposition of xylan, the main component of hemicellulose, was measured and evaluated by the method shown below ( 3) Decomposing activity of 4-nitrophenyl-β-D-xylopyranoside (pNP-Xyl). In addition, the substrates of the above (1) to (3) are collectively referred to as pNP-sugar.

[0101] 0.1 mL of enzyme solution was added to 0.9 mL of 100 mM aceta...

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Abstract

The method of preparing a sugar solution according to the present invention is a method of preparing a sugar solution from cellulose-containing biomass, the method including step (1): a step for obtaining an endoxylanase hydrolysate by hydrolyzing the cellulose-containing biomass using endoxylanase derived from microorganisms of the Acremonium genus or the Aspergillus genus, step (2): a step for separating the endoxylanase hydrolysate into an endoxylanase hydrolysate solid and an endoxylanase hydrolysate liquid through solid-liquid separation, step (3): a step for obtaining a cellulase hydrolysate by hydrolyzing the endoxylanase hydrolysate solid using cellulase derived from a filamentous fungus, and step (4): a step for filtering the cellulase hydrolysate through an ultrafiltration membrane to collect a sugar solution from the filtrate side and collect an enzyme component from the non-filtrate side.

Description

technical field [0001] The present invention relates to a method for producing a sugar liquid that can be used as a fermentation raw material and the like from biomass. Background technique [0002] Fermentative production processes of sugar-based chemicals are used in the production of various industrial raw materials. As the sugar used as the raw material for this fermentation, sugar derived from edible raw materials such as sugar cane, starch, and sugar beet is currently used industrially. However, in view of the rising price of edible raw materials due to the increase in the world population in the future, or the ethical aspects of competition with food, it is necessary to construct a process for efficiently producing sugar liquid from renewable non-edible resources, that is, cellulose-containing biomass, or The process of using the obtained sugar liquid as a fermentation raw material and efficiently converting it into an industrial raw material will become a future iss...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14
CPCC12P19/14C12P19/02C12Y302/01004C12Y302/01008Y02E50/10
Inventor 船田茂行栗原宏征山田胜成
Owner TORAY IND INC
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