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Mycobacterium bovis and brucella abortus dual detection card and preparation method thereof

A technology of Brucella bovis and Mycobacterium, which is applied in the directions of measuring devices, analytical materials, instruments, etc., to achieve the effects of intuitive display, easy operation and fast interpretation.

Inactive Publication Date: 2017-05-24
深圳市绿诗源生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some detection methods in the field of bovine tuberculosis and bovine brucellosis immunoserological antibody detection are only for the detection of one disease

Method used

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  • Mycobacterium bovis and brucella abortus dual detection card and preparation method thereof
  • Mycobacterium bovis and brucella abortus dual detection card and preparation method thereof
  • Mycobacterium bovis and brucella abortus dual detection card and preparation method thereof

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Embodiment 1

[0053] A dual detection card for Mycobacterium bovis and Brucella bovis provided by the invention, see figure 1 , including a housing 1, the housing 1 is provided with a sampling hole 2 and an observation window 3, and a colloidal gold test strip is provided in the housing 1, and it is characterized in that the colloidal gold test strip includes a base plate 4, The bottom plate 4 is successively connected with a sample pad 5, a gold standard pad 6, a coating film 7 and a water-absorbing pad 8; the coating film 7 is provided with a Mycobacterium tuberculosis membrane protein MPB70-MPB83 detection line T1, a Brucella bovis LPS detection line T2 and goat anti-rabbit polyclonal antibody quality control line C, these three lines are arranged in parallel.

[0054] Specifically, the two ends of the coating film 7 are respectively overlapped with the water-absorbing pad 8 and the gold standard pad 6, and the sample pad 5 is pressed on the gold standard pad 6, and the sample pad 5 is a...

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Abstract

The invention discloses a mycobacterium bovis and brucella abortus dual detection card and a preparation method thereof and aims to provide a detection card which is capable of simultaneously detecting mycobacterium bovis and brucella abortus in a bovine serum specimen, is short in detection time, high in stability, simple in operation and intuitive and reliable in result judgment and does not need other instruments or professional personnel. According to the technical key points, the detection card comprises a shell (1), wherein a sample adding hole (2) and an observation window (3) are formed in the shell (1); and a colloidal gold test strip is arranged in the shell (1). The detection card is characterized in that the colloidal gold test strip comprises a bottom plate (4); a sample pad (5), a gold label pad (6), a coating membrane (7) and an absorbent pad (8) are sequentially connected onto the bottom plate (4); a mycobacterium bovis membrane protein MPB70-MPB83 detection line T1, a brucella abortus LPS detection line T2 and a goat anti-rabbit polyclonal antibody quality control line C are formed in the coating membrane (7); and the three lines are arranged in parallel. The detection card belongs to the technical field of biology.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a dual detection card for Mycobacterium bovis and Brucella bovis and a preparation method thereof. Background technique [0002] Bovine tuberculosis is a zoonotic chronic wasting infectious disease caused by Mycobacterium bovis. It will cause serious harm to the dairy industry and the health of humans and animals. [0003] Bovine brucellosis is a zoonotic chronic bacterial infectious disease caused by Brucella bovis (B. abortus). Infected animals and humans often cause damage to the reproductive system, pose a serious threat to human physical and mental health and cause huge economic losses to the livestock industry. [0004] Detection of bovine Mycobacterium bovis or Brucella antibody in bovine serum is one of the immunological serological diagnosis methods for bovine tuberculosis or bovine brucellosis. Chinese patents CN200910218156.6 and CN200610166551 respectively ...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/559
CPCG01N33/559G01N33/56911G01N33/5695
Inventor 刘海森蒋永青刘晶张金超黄振宇
Owner 深圳市绿诗源生物技术有限公司
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